Yields from glucose, xylose, and paper sludge hydrolysate during hydrogen production by the extreme thermophile Caldicellulosiruptor saccharolyticus

Zsófia Kádár, Truus De Vrije, Giel E. Van Noorden, Miriam A.W. Budde, Zsolt Szengyel, Kati Réczey, Pieternel A.M. Claassen

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

This study addressed the utilization of an industrial waste stream, paper sludge, as a renewable cheap feedstock for the fermentative production of hydrogen by the extreme thermophile Caldicellulosiruptor saccharolyticus. Hydrogen, acetate, and lactate were produced in medium in which paper sludge hydrolysate was added as the sole carbon and energy source and in control medium with the same concentration of analytical grade glucose and xylose. The hydrogen yield was dependent on lactate formation and varied between 50 and 94% of the theoretical maximum. The carbon balance in the medium with glucose and xylose was virtually 100%. The carbon balance was not complete in the paper sludge medium because the measurement of biomass was impaired owing to interfering components in the paper sludge hydrolysate. Nevertheless, >85% of the carbon could be accounted for in the products acetate and lactate. The maximal volumetric hydrogen production rate was 5 to 6 mmol/(L·h), which was lower than the production rate in media with glucose, xylose, or a combination of these sugars (9-11 mmol/ [L·h]). The reduced hydrogen production rate suggests the presence of inhibiting components in paper sludge hydrolysate.

Original languageEnglish
Pages (from-to)497-508
Number of pages12
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume114
Issue number1-3
DOIs
Publication statusPublished - Mar 1 2004

Keywords

  • Caldicellulosiruptor saccharolyticus, glucose
  • Carbon balances
  • Extreme thermophile
  • Hydrogen production
  • Paper sludge hydrolysate
  • Xylose

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

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