Yeast-like cell formation and glutathione metabolism in autolysing cultures of Penicillium chrysogenum

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Abstract

The bulk formation of yeast-like (arthrospore-like) cells were typical in carbon-depleted submerged cultures of the high β-lactam producer Penicillium chrysogenum NCAIM 00237 strain independently of the nitrogen-content of the culture medium. This morphogenetic switch was still quite common in carbonstarving cultures of the low-penicillin-producer strain P. chrysogenum ATCC 28089 (Wis 54-1255) when the nitrogen-content of the medium was low but was a very rare event in wild-type P. chrysogenum cultures. The mycelium→yeast-like cell transition correlated well with a relatively high glutathione concentration and a reductive glutathione/glutathione disulfite (GSH/GSSG) redox balance in autolysing cultures, which was a consequence of industrial strain development. Paradoxically, the development of high β-lactam productivity resulted in a high intracellular GSH level and, concomitantly, in an increased γ-glutamyltranspeptidase (i.e. GSH-decomposing) activity in the autolytic phase of growth of P. chrysogenum NCAIM 00237. The hypothesized causal connection between GSH metabolism and cell morphology, if verified, may help us in future metabolic engineering of industrially important filamentous fungi.

Original languageEnglish
Pages (from-to)431-440
Number of pages10
JournalActa Biologica Hungarica
Volume58
Issue number4
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Penicillium chrysogenum
Metabolism
Yeast
yeast
Glutathione
glutathione
Lactams
Yeasts
metabolism
lactams
yeasts
Nitrogen
nitrogen content
Metabolic engineering
Glutathione Disulfide
cells
Fungi
Metabolic Engineering
Penicillins
metabolic engineering

Keywords

  • Acremonium chrysogenum
  • Arthrospores
  • Cell death
  • Fragmentation
  • Glutathione status
  • Morphology
  • Penicillium chrysogenum
  • Yeast-like cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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title = "Yeast-like cell formation and glutathione metabolism in autolysing cultures of Penicillium chrysogenum",
abstract = "The bulk formation of yeast-like (arthrospore-like) cells were typical in carbon-depleted submerged cultures of the high β-lactam producer Penicillium chrysogenum NCAIM 00237 strain independently of the nitrogen-content of the culture medium. This morphogenetic switch was still quite common in carbonstarving cultures of the low-penicillin-producer strain P. chrysogenum ATCC 28089 (Wis 54-1255) when the nitrogen-content of the medium was low but was a very rare event in wild-type P. chrysogenum cultures. The mycelium→yeast-like cell transition correlated well with a relatively high glutathione concentration and a reductive glutathione/glutathione disulfite (GSH/GSSG) redox balance in autolysing cultures, which was a consequence of industrial strain development. Paradoxically, the development of high β-lactam productivity resulted in a high intracellular GSH level and, concomitantly, in an increased γ-glutamyltranspeptidase (i.e. GSH-decomposing) activity in the autolytic phase of growth of P. chrysogenum NCAIM 00237. The hypothesized causal connection between GSH metabolism and cell morphology, if verified, may help us in future metabolic engineering of industrially important filamentous fungi.",
keywords = "Acremonium chrysogenum, Arthrospores, Cell death, Fragmentation, Glutathione status, Morphology, Penicillium chrysogenum, Yeast-like cells",
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T1 - Yeast-like cell formation and glutathione metabolism in autolysing cultures of Penicillium chrysogenum

AU - Pócsi, I.

AU - Molnár, Z.

AU - Pusztahelyi, T.

AU - Varecza, Z.

AU - Emri, T.

PY - 2007/12

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N2 - The bulk formation of yeast-like (arthrospore-like) cells were typical in carbon-depleted submerged cultures of the high β-lactam producer Penicillium chrysogenum NCAIM 00237 strain independently of the nitrogen-content of the culture medium. This morphogenetic switch was still quite common in carbonstarving cultures of the low-penicillin-producer strain P. chrysogenum ATCC 28089 (Wis 54-1255) when the nitrogen-content of the medium was low but was a very rare event in wild-type P. chrysogenum cultures. The mycelium→yeast-like cell transition correlated well with a relatively high glutathione concentration and a reductive glutathione/glutathione disulfite (GSH/GSSG) redox balance in autolysing cultures, which was a consequence of industrial strain development. Paradoxically, the development of high β-lactam productivity resulted in a high intracellular GSH level and, concomitantly, in an increased γ-glutamyltranspeptidase (i.e. GSH-decomposing) activity in the autolytic phase of growth of P. chrysogenum NCAIM 00237. The hypothesized causal connection between GSH metabolism and cell morphology, if verified, may help us in future metabolic engineering of industrially important filamentous fungi.

AB - The bulk formation of yeast-like (arthrospore-like) cells were typical in carbon-depleted submerged cultures of the high β-lactam producer Penicillium chrysogenum NCAIM 00237 strain independently of the nitrogen-content of the culture medium. This morphogenetic switch was still quite common in carbonstarving cultures of the low-penicillin-producer strain P. chrysogenum ATCC 28089 (Wis 54-1255) when the nitrogen-content of the medium was low but was a very rare event in wild-type P. chrysogenum cultures. The mycelium→yeast-like cell transition correlated well with a relatively high glutathione concentration and a reductive glutathione/glutathione disulfite (GSH/GSSG) redox balance in autolysing cultures, which was a consequence of industrial strain development. Paradoxically, the development of high β-lactam productivity resulted in a high intracellular GSH level and, concomitantly, in an increased γ-glutamyltranspeptidase (i.e. GSH-decomposing) activity in the autolytic phase of growth of P. chrysogenum NCAIM 00237. The hypothesized causal connection between GSH metabolism and cell morphology, if verified, may help us in future metabolic engineering of industrially important filamentous fungi.

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