Xenotransplantation of human intestine into mouse abdomen or subcutaneous tissue: Novel platforms for the study of the human enteric nervous system

N. Nagy, N. Marsiano, R. S. Bruckner, M. Scharl, M. J. Gutnick, S. Yagel, E. Arciero, A. M. Goldstein, N. Y. Shpigel

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Abstract

Background: Current efforts to develop stem cell therapy as a novel treatment for neurointestinal diseases are limited by the unavailability of a model system to study cell transplantation in the human intestine. We propose that xenograft models support enteric nervous system (ENS) development in the fetal human intestine when transplanted into mice subcutaneously or intra-abdominally. Methods: Fetal human small and large intestine were grafted onto the small intestinal mesentery and into the subcutaneous tissue of immunodeficient mice for up to 4 months. Intestinal cytoarchitecture and ENS development were studied using immunohistochemistry. Key Results: In both abdominal and subcutaneous grafts, the intestine developed normally with formation of mature epithelial and mesenchymal layers. The ENS was patterned in two ganglionated plexuses containing enteric neurons and glia, including cholinergic and nitrergic neuronal subtypes. c-Kit-immunoreactive interstitial cells of Cajal were present in the gut wall. Conclusions & Inferences: Abdominal xenografts represent a novel model that supports the growth and development of fetal human intestine. This in vivo approach will be a useful method to study maturation of the ENS, the pathophysiology of neurointestinal diseases, and the long-term survival and functional differentiation of neuronal stem cells for the treatment of enteric neuropathies.

Original languageEnglish
Article numbere13212
JournalNeurogastroenterology and Motility
Volume30
Issue number3
DOIs
Publication statusPublished - Mar 2018

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Keywords

  • enteric nervous system
  • enteric neuropathies
  • fetal human intestine
  • xenotransplant

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

Cite this

Nagy, N., Marsiano, N., Bruckner, R. S., Scharl, M., Gutnick, M. J., Yagel, S., Arciero, E., Goldstein, A. M., & Shpigel, N. Y. (2018). Xenotransplantation of human intestine into mouse abdomen or subcutaneous tissue: Novel platforms for the study of the human enteric nervous system. Neurogastroenterology and Motility, 30(3), [e13212]. https://doi.org/10.1111/nmo.13212