Xenorhabdus antibiotics: A comparative analysis and potential utility for controlling mastitis caused by bacteria

G. Furgani, E. Böszörményi, A. Fodor, A. Máthé-Fodor, S. Forst, J. S. Hogan, Z. Katona, M. G. Klein, E. Stackebrandt, A. Szentirmai, F. Sztaricskai, S. L. Wolf

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Aims: The role of antibiotics produced by bacterial symbionts of entomopathogenic nematodes is to suppress growth of microbes in the soil environment. These antibiotics are active against Gram-positive and Gram-negative bacteria, and were tested against mastitis isolates from dairy cows. Methods and Results: Two bioassays were adapted for Xenorhabdus antibiotics; an overlay method on agar plates, and serially diluted, cell-free, Xenorhabdus cultures. The antimicrobial activities of the liquid cultures of 13 strains from five Xenorhabdus species were further evaluated. Antimicrobial activities of the type strains of X. nematophila, X. budapestensis and X. szentirmaii were tested on mastitis isolates of Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae with both bioassays. A previously reported antibiotic from X. nematophila, nematophin, was synthesized in three steps from tryptamine and 4-methyl-2-oxovaleric acid sodium salt. Conclusions: The antibiotics of all three Xenorhabdus strains were powerful in either bioassay, but the sensitivity of the isolates differed from each other. While Kl. pneumoniae was the least susceptible, Staph. aureus had the highest sensitivity to each Xenorhabdus strain. Xenorhabdus szentirmaii and X. budapestensis were more potent antibiotic producers than X. nematophila, and raceme nematophin was ineffective against all mastitis isolates. Significance and Impact of the Study: These results indicate that Xenorhabdus antibiotics are effective against mastitis isolates and should be further evaluated for their potential in mastitis control or prevention.

Original languageEnglish
Pages (from-to)745-758
Number of pages14
JournalJournal of Applied Microbiology
Volume104
Issue number3
DOIs
Publication statusPublished - Mar 2008

Fingerprint

Xenorhabdus
Mastitis
mastitis
antibiotics
Anti-Bacterial Agents
Bacteria
bacteria
Xenorhabdus nematophila
Biological Assay
bioassays
Klebsiella pneumoniae
anti-infective agents
tryptamine
entomopathogenic nematodes
edaphic factors
Gram-Negative Bacteria
Gram-negative bacteria
symbionts
Agar
Staphylococcus aureus

Keywords

  • Entomopathogenic nematodes/bacteria
  • Escherichia coli
  • Klebsiella pneumoniae
  • Mastitis isolates
  • Nematophin
  • Staphylococcus aureus
  • Xenorhabdus antibiotics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Xenorhabdus antibiotics : A comparative analysis and potential utility for controlling mastitis caused by bacteria. / Furgani, G.; Böszörményi, E.; Fodor, A.; Máthé-Fodor, A.; Forst, S.; Hogan, J. S.; Katona, Z.; Klein, M. G.; Stackebrandt, E.; Szentirmai, A.; Sztaricskai, F.; Wolf, S. L.

In: Journal of Applied Microbiology, Vol. 104, No. 3, 03.2008, p. 745-758.

Research output: Contribution to journalArticle

Furgani, G, Böszörményi, E, Fodor, A, Máthé-Fodor, A, Forst, S, Hogan, JS, Katona, Z, Klein, MG, Stackebrandt, E, Szentirmai, A, Sztaricskai, F & Wolf, SL 2008, 'Xenorhabdus antibiotics: A comparative analysis and potential utility for controlling mastitis caused by bacteria', Journal of Applied Microbiology, vol. 104, no. 3, pp. 745-758. https://doi.org/10.1111/j.1365-2672.2007.03613.x
Furgani, G. ; Böszörményi, E. ; Fodor, A. ; Máthé-Fodor, A. ; Forst, S. ; Hogan, J. S. ; Katona, Z. ; Klein, M. G. ; Stackebrandt, E. ; Szentirmai, A. ; Sztaricskai, F. ; Wolf, S. L. / Xenorhabdus antibiotics : A comparative analysis and potential utility for controlling mastitis caused by bacteria. In: Journal of Applied Microbiology. 2008 ; Vol. 104, No. 3. pp. 745-758.
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abstract = "Aims: The role of antibiotics produced by bacterial symbionts of entomopathogenic nematodes is to suppress growth of microbes in the soil environment. These antibiotics are active against Gram-positive and Gram-negative bacteria, and were tested against mastitis isolates from dairy cows. Methods and Results: Two bioassays were adapted for Xenorhabdus antibiotics; an overlay method on agar plates, and serially diluted, cell-free, Xenorhabdus cultures. The antimicrobial activities of the liquid cultures of 13 strains from five Xenorhabdus species were further evaluated. Antimicrobial activities of the type strains of X. nematophila, X. budapestensis and X. szentirmaii were tested on mastitis isolates of Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae with both bioassays. A previously reported antibiotic from X. nematophila, nematophin, was synthesized in three steps from tryptamine and 4-methyl-2-oxovaleric acid sodium salt. Conclusions: The antibiotics of all three Xenorhabdus strains were powerful in either bioassay, but the sensitivity of the isolates differed from each other. While Kl. pneumoniae was the least susceptible, Staph. aureus had the highest sensitivity to each Xenorhabdus strain. Xenorhabdus szentirmaii and X. budapestensis were more potent antibiotic producers than X. nematophila, and raceme nematophin was ineffective against all mastitis isolates. Significance and Impact of the Study: These results indicate that Xenorhabdus antibiotics are effective against mastitis isolates and should be further evaluated for their potential in mastitis control or prevention.",
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AU - Forst, S.

AU - Hogan, J. S.

AU - Katona, Z.

AU - Klein, M. G.

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AU - Wolf, S. L.

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