X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats

Renáta Fábián, A. Kovács, Viktor Stéger, Krisztián Frank, I. Egerszegi, János Oláh, Szilárd Bodó

Research output: Contribution to journalArticle

1 Citation (Scopus)


The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PISaffected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.

Original languageEnglish
Pages (from-to)500-504
Number of pages5
JournalActa Veterinaria Hungarica
Issue number4
Publication statusPublished - Dec 1 2017



  • Amelogenin
  • Goat
  • Intersex
  • Polled Intersex Syndrome
  • Sex determination

ASJC Scopus subject areas

  • veterinary(all)

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