WPMLG-5 spectroscopy of self-aggregated BChl e in natural chlorosomes of Chlorobaculum Limnaeum

Y. Miloslavina, Karthick Babu Sai Sankar Gupta, Marcus Tank, Donald A. Bryant, Huub J M De Groot

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

1H magic angle spinning (MAS) nuclear magnetic resonance (NMR), employing rapid spinning and the wPMLG-5 pulse sequence, was used to explore the possibility for performing 1H solid state NMR of a light-harvesting organelle, the chlorosome antenna of Chlorobaculum limnaeum. This natural antenna system is built from bacteriochlorophyll e (BChl e) molecules that are self-assembled to form a supramolecular scaffold for in vivo harvesting of light. We present preliminary data on this chlorosome species and address the feasibility of performing wPMLG spectroscopy, in terms of high power irradiation on a fragile biological sample. In parallel, enhancing the 1H shift dispersion from the magnetic field can help to resolve signals from protons that resonate downfield. Different line narrowing mechanisms operating in parallel provide access to resolving selected 1H signals collected from the moderately sized and chemically diverse BChl e molecular motif in the chlorosome scaffold. These discoveries will be helpful for future studies of structural and functional characteristics of self-assembled natural and artificial light-harvesting molecules.

Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalIsrael Journal of Chemistry
Volume54
Issue number1-2
DOIs
Publication statusPublished - 2014

Fingerprint

Spectroscopy
Scaffolds
Nuclear magnetic resonance
Antennas
Forms (concrete)
Magic angle spinning
Molecules
Protons
Irradiation
Magnetic fields
bacteriochlorophyll e

Keywords

  • Bacteriochlorophyll e
  • Chlorosomes
  • Light-harvesting
  • MAS NMR spectroscopy
  • Photosynthesis
  • WPMLG

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

WPMLG-5 spectroscopy of self-aggregated BChl e in natural chlorosomes of Chlorobaculum Limnaeum. / Miloslavina, Y.; Gupta, Karthick Babu Sai Sankar; Tank, Marcus; Bryant, Donald A.; De Groot, Huub J M.

In: Israel Journal of Chemistry, Vol. 54, No. 1-2, 2014, p. 147-153.

Research output: Contribution to journalArticle

Miloslavina, Y. ; Gupta, Karthick Babu Sai Sankar ; Tank, Marcus ; Bryant, Donald A. ; De Groot, Huub J M. / WPMLG-5 spectroscopy of self-aggregated BChl e in natural chlorosomes of Chlorobaculum Limnaeum. In: Israel Journal of Chemistry. 2014 ; Vol. 54, No. 1-2. pp. 147-153.
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