Wound-healing markers revealed by proximity extension assay in tears of patients following glaucoma surgery

Éva Csősz, Noémi Tóth, Eszter Deák, Adrienne Csutak, J. Tőzsér

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Tears are a constantly available and highly valuable body fluid collectable by non-invasive techniques. Although it can give information on ocular status and be used for follow-ups, tear analysis is challenging due to the low amount of sample that is available. Proximity extension assay (PEA) allows for a sensitive and scalable analysis of multiple proteins in a single run from a one-µL sample, so we applied this technique and examined the amount of 184 proteins in tears collected at different time points after trabeculectomy. The success rate of this surgical intervention highly depends on proper wound healing; therefore, information on the process is indispensable. We observed significantly higher levels of IL-6 and MMP1 at the early time points (day one, two, and four) following trabeculectomy, and the protein amounts went back to the level observed before the surgery three months after the intervention. Patients with or without complications were tested, and proteins that have roles in the immune response and wound healing could be observed with altered frequency and amounts in the cases of patients with complications. Our results highlight the importance of inflammation in wound-healing complications, and at the same time, indicate the utility of PEA in tear analysis.

Original languageEnglish
Article number4096
JournalInternational Journal of Molecular Sciences
Volume19
Issue number12
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

glaucoma
wound healing
Tears
surgery
Glaucoma
markers
Wound Healing
Surgery
proximity
Assays
Trabeculectomy
proteins
Proteins
Body Fluids
body fluids
Interleukin-6
Body fluids
healing
Inflammation

Keywords

  • Glaucoma
  • Inflammation
  • Proximity extension assay
  • Tear
  • Wound healing

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Wound-healing markers revealed by proximity extension assay in tears of patients following glaucoma surgery. / Csősz, Éva; Tóth, Noémi; Deák, Eszter; Csutak, Adrienne; Tőzsér, J.

In: International Journal of Molecular Sciences, Vol. 19, No. 12, 4096, 01.01.2018.

Research output: Contribution to journalArticle

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