Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex

Michael Boitard, Riccardo Bocchi, Kristof Egervari, Volodymyr Petrenko, Beatrice Viale, Stéphane Gremaud, Eloisa Zgraggen, Patrick Salmon, J. Kiss

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The precise timing of pyramidal cell migration from the ventricular germinal zone to the cortical plate is essential for establishing cortical layers, and migration errors can lead to neurodevelopmental disorders underlying psychiatric and neurological diseases. Here, we report that Wnt canonical as well as non-canonicalsignaling is active in pyramidal precursorsduring radial migration. We demonstrate using constitutive and conditional genetic strategies that transient downregulation of canonical Wnt/β-catenin signaling during the multipolar stage plays a critical role in polarizing and orienting cells for radial migration. In addition, we show that reduced canonical Wnt signaling is triggered cell autonomously by time-dependent expression of Wnt5A and activation of non-canonical signaling. We identify ephrin-B1 as a canonical Wnt-signaling-regulated target in control of the multipolar-to-bipolar switch. These findings highlight the critical role of Wnt signaling activity in neuronal positioning during cortical development.

Original languageEnglish
Pages (from-to)1349-1361
Number of pages13
JournalCell Reports
Volume10
Issue number8
DOIs
Publication statusPublished - Mar 3 2015

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Cerebral Cortex
Neurons
Cell Movement
Ephrin-B1
Catenins
Pyramidal Cells
Psychiatry
Down-Regulation
Chemical activation
Switches
Neurodevelopmental Disorders

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex. / Boitard, Michael; Bocchi, Riccardo; Egervari, Kristof; Petrenko, Volodymyr; Viale, Beatrice; Gremaud, Stéphane; Zgraggen, Eloisa; Salmon, Patrick; Kiss, J.

In: Cell Reports, Vol. 10, No. 8, 03.03.2015, p. 1349-1361.

Research output: Contribution to journalArticle

Boitard, M, Bocchi, R, Egervari, K, Petrenko, V, Viale, B, Gremaud, S, Zgraggen, E, Salmon, P & Kiss, J 2015, 'Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex', Cell Reports, vol. 10, no. 8, pp. 1349-1361. https://doi.org/10.1016/j.celrep.2015.01.061
Boitard, Michael ; Bocchi, Riccardo ; Egervari, Kristof ; Petrenko, Volodymyr ; Viale, Beatrice ; Gremaud, Stéphane ; Zgraggen, Eloisa ; Salmon, Patrick ; Kiss, J. / Wnt signaling regulates multipolar-to-bipolar transition of migrating neurons in the cerebral cortex. In: Cell Reports. 2015 ; Vol. 10, No. 8. pp. 1349-1361.
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