Widespread presence of bovine proteins in human cell lines

Simon Sugár, Lilla Turiák, Károly Vékey, L. Drahos

Research output: Contribution to journalArticle


HPLC-MS/MS analysis of various human cell lines shows the presence of a major amount of bovine protein contaminants. These likely originate from fetal bovine serum (FBS), typically used in cell cultures. If evaluated against a human protein database, on average 10% of the identified human proteins will be misleading (bovine proteins, but indicated as if they were human). Bovine contaminants therefore may cause major bias in proteomic studies of cell cultures, if not considered explicitly.

Original languageEnglish
Article numbere4464
JournalJournal of Mass Spectrometry
Issue number7
Publication statusPublished - Jul 1 2020

ASJC Scopus subject areas

  • Spectroscopy

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