Widespread alterations in the synaptic proteome of the adolescent cerebral cortex following prenatal immune activation in rats

B. Györffy, Péter Gulyássy, Barbara Gellén, Katalin Völgyi, Dóra Madarasi, Viktor Kis, Olivér Ozohanics, Ildikó Papp, Péter Kovács, Gert Lubec, A. Dobolyi, J. Kardos, L. Drahos, G. Juhász, K. Kékesi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An increasing number of studies have revealed associations between pre- and perinatal immune activation and the development of schizophrenia and autism spectrum disorders (ASDs). Accordingly, neuroimmune crosstalk has a considerably large impact on brain development during early ontogenesis. While a plethora of heterogeneous abnormalities have already been described in established maternal immune activation (MIA) rodent and primate animal models, which highly correlate to those found in human diseases, the underlying molecular background remains obscure. In the current study, we describe the long-term effects of MIA on the neocortical pre- and postsynaptic proteome of adolescent rat offspring in detail. Molecular differences were revealed in sub-synaptic fractions, which were first thoroughly characterized using independent methods. The widespread proteomic examination of cortical samples from offspring exposed to maternal lipopolysaccharide administration at embryonic day 13.5 was conducted via combinations of different gel-based proteomic techniques and tandem mass spectrometry. Our experimentally validated proteomic data revealed more pre- than postsynaptic protein level changes in the offspring. The results propose the relevance of altered synaptic vesicle recycling, cytoskeletal structure and energy metabolism in the presynaptic region in addition to alterations in vesicle trafficking, the cytoskeleton and signal transduction in the postsynaptic compartment in MIA offspring. Differing levels of the prominent signaling regulator molecule calcium/calmodulin-dependent protein kinase II in the postsynapse was validated and identified specifically in the prefrontal cortex. Finally, several potential common molecular regulators of these altered proteins, which are already known to be implicated in schizophrenia and ASD, were identified and assessed. In summary, unexpectedly widespread changes in the synaptic molecular machinery in MIA rats were demonstrated which might underlie the pathological cortical functions that are characteristic of schizophrenia and ASD.

Original languageEnglish
JournalBrain, Behavior, and Immunity
DOIs
Publication statusAccepted/In press - Jan 27 2016

Fingerprint

Proteome
Cerebral Cortex
Mothers
Proteomics
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Schizophrenia
Calcium-Calmodulin-Dependent Protein Kinases
Synaptic Vesicles
Tandem Mass Spectrometry
Prefrontal Cortex
Cytoskeleton
Energy Metabolism
Primates
Lipopolysaccharides
Rodentia
Signal Transduction
Proteins
Animal Models
Gels
Brain

Keywords

  • Autism spectrum disorder (ASD)
  • Cerebral cortex
  • Maternal immune activation (MIA)
  • Neuroproteomics
  • Schizophrenia
  • Synaptosome

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Widespread alterations in the synaptic proteome of the adolescent cerebral cortex following prenatal immune activation in rats. / Györffy, B.; Gulyássy, Péter; Gellén, Barbara; Völgyi, Katalin; Madarasi, Dóra; Kis, Viktor; Ozohanics, Olivér; Papp, Ildikó; Kovács, Péter; Lubec, Gert; Dobolyi, A.; Kardos, J.; Drahos, L.; Juhász, G.; Kékesi, K.

In: Brain, Behavior, and Immunity, 27.01.2016.

Research output: Contribution to journalArticle

Györffy, B. ; Gulyássy, Péter ; Gellén, Barbara ; Völgyi, Katalin ; Madarasi, Dóra ; Kis, Viktor ; Ozohanics, Olivér ; Papp, Ildikó ; Kovács, Péter ; Lubec, Gert ; Dobolyi, A. ; Kardos, J. ; Drahos, L. ; Juhász, G. ; Kékesi, K. / Widespread alterations in the synaptic proteome of the adolescent cerebral cortex following prenatal immune activation in rats. In: Brain, Behavior, and Immunity. 2016.
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