Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment

Peter Tompa, Kyou Hoon Han, M. Bokor, Pawel Kamasa, Ágnes Tantos, Beáta Fritz, Do Hyoung Kim, Chewook Lee, Tamás Verebélyi, K. Tompa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Wide-line 1H NMR intensity and differential scanning calorimetry measurements were carried out on the intrinsically disordered 73-residue full transactivation domain (TAD) of the p53 tumor suppressor protein and two peptides: one a wild type p53 TAD peptide with a helix pre-structuring property, and a mutant peptide with a disabled helix-forming propensity. Measurements were carried out in order to characterize their water and ion binding characteristics. By quantifying the number of hydrate water molecules, we provide a microscopic description for the interactions of water with a wild-type p53 TAD and two p53 TAD peptides. The results provide direct evidence that intrinsically disordered proteins (IDPs) and a less structured peptide not only have a higher hydration capacity than globular proteins, but are also able to bind a larger amount of charged solute ions.

Original languageEnglish
Pages (from-to)497-501
Number of pages5
JournalBMB Reports
Volume49
Issue number9
DOIs
Publication statusPublished - 2016

Fingerprint

Transcriptional Activation
Nuclear magnetic resonance
Peptides
Water
Intrinsically Disordered Proteins
Ions
Tumor Suppressor Protein p53
Differential Scanning Calorimetry
Hydrates
Hydration
Differential scanning calorimetry
Molecules
Proteins
Protein Domains

Keywords

  • Differential Scanning Calorimetry (DSC)
  • Hydration
  • P53 Transactivation Domain (p53TAD)
  • Pre-Structured Motif (PreSMo)
  • Wide-line NMR

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment. / Tompa, Peter; Han, Kyou Hoon; Bokor, M.; Kamasa, Pawel; Tantos, Ágnes; Fritz, Beáta; Kim, Do Hyoung; Lee, Chewook; Verebélyi, Tamás; Tompa, K.

In: BMB Reports, Vol. 49, No. 9, 2016, p. 497-501.

Research output: Contribution to journalArticle

Tompa, Peter ; Han, Kyou Hoon ; Bokor, M. ; Kamasa, Pawel ; Tantos, Ágnes ; Fritz, Beáta ; Kim, Do Hyoung ; Lee, Chewook ; Verebélyi, Tamás ; Tompa, K. / Wide-line NMR and DSC studies on intrinsically disordered p53 transactivation domain and its helically pre-structured segment. In: BMB Reports. 2016 ; Vol. 49, No. 9. pp. 497-501.
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AU - Tantos, Ágnes

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