Whole genome characterization of a chelonian orthoreovirus strain identifies significant genetic diversity and may classify reptile orthoreoviruses into distinct species

Renáta Kugler, Rachel E. Marschang, Katalin Ihász, György Lengyel, F. Jakab, K. Bányai, Szilvia L. Farkas

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study we report the sequence and phylogenetic characterization of an orthoreovirus strain, CH1197/96, isolated from a spur-thighed tortoise (Testudo graeca) on chicken embryo fibroblast cells. The 23,957 bp long genome sequence was obtained by combined use of semiconductor and capillary sequencing. Although the genomic characterization showed that the virus was most similar to the bush viper reovirus strain, 47/02, and in phylogenies performed with all segments the two strains formed a monophyletic group, the nucleotide (48.4-70.3%) and amino acid (39.2-80.7%) sequence identity values were moderate between the two reptile origin reoviruses. Based on our results and existing classification criteria for the genus Orthoreovirus, the tortoise reovirus strain CH1197/96 might be the first representative of a novel reptilian origin Orthoreovirus species.

Original languageEnglish
Pages (from-to)94-98
Number of pages5
JournalVirus Research
Volume215
DOIs
Publication statusPublished - Apr 2 2016

Fingerprint

Orthoreovirus
Reptiles
Turtles
Genome
Semiconductors
Phylogeny
Chickens
Embryonic Structures
Nucleotides
Fibroblasts
Viruses
Amino Acids

Keywords

  • Orthoreovirus
  • Phylogenetic analysis
  • Reoviridae
  • Testudo graeca
  • Virus taxonomy

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Whole genome characterization of a chelonian orthoreovirus strain identifies significant genetic diversity and may classify reptile orthoreoviruses into distinct species. / Kugler, Renáta; Marschang, Rachel E.; Ihász, Katalin; Lengyel, György; Jakab, F.; Bányai, K.; Farkas, Szilvia L.

In: Virus Research, Vol. 215, 02.04.2016, p. 94-98.

Research output: Contribution to journalArticle

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