Whole blood samples from Alzheimer patients and control donors demonstrate fluorimetric differences

Andràs Palotàs, Jànos Kàlmàn, Miklós Palotàs, Keyvan Matin, Károly Szentpáli, Attila Paszt, Zoltán Janka, Botond Penke

Research output: Contribution to journalArticle

Abstract

β-amyloid peptide plays a crucial role in the pathology of Alzheimer's disease. As part of our ongoing fluorimetric studies, in the present report we demonstrate differences in resting intracellular free calcium levels of cells in the blood derived from sporadic Alzheimer patients and from age-matched control individuals. Calcium levels were measured in Fura-2AM-loaded human blood samples by dual-wavelength spectrofluorimetry. The resting calcium concentrations of blood samples from Alzheimer patients were lower compared to that of the control samples. Exposure of control blood samples to β-amyloid caused an increase in the calcium level. Specimens from Alzheimer donors, however, appeared to be resistant to the peptide. This simple finding may serve as a springboard to monitoring Alzheimer pathology in the peripheral systems of the body.

Original languageEnglish
Pages (from-to)1589-1591
Number of pages3
JournalNeurochemical research
Volume27
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

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Keywords

  • Alzheimer's disease
  • Blood
  • Fluorescence
  • β-amyloid

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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