Whole blood samples from Alzheimer patients and control donors demonstrate fluorimetric differences

Andràs Palotàs, J. Kálmán, Miklós Palotàs, Keyvan Matin, Károly Szentpáli, Attila Paszt, Z. Janka, B. Penke

Research output: Contribution to journalArticle

Abstract

β-amyloid peptide plays a crucial role in the pathology of Alzheimer's disease. As part of our ongoing fluorimetric studies, in the present report we demonstrate differences in resting intracellular free calcium levels of cells in the blood derived from sporadic Alzheimer patients and from age-matched control individuals. Calcium levels were measured in Fura-2AM-loaded human blood samples by dual-wavelength spectrofluorimetry. The resting calcium concentrations of blood samples from Alzheimer patients were lower compared to that of the control samples. Exposure of control blood samples to β-amyloid caused an increase in the calcium level. Specimens from Alzheimer donors, however, appeared to be resistant to the peptide. This simple finding may serve as a springboard to monitoring Alzheimer pathology in the peripheral systems of the body.

Original languageEnglish
Pages (from-to)1589-1591
Number of pages3
JournalNeurochemical Research
Volume27
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

Fingerprint

Blood
Tissue Donors
Calcium
Pathology
Amyloid
Peptides
Blood Cells
Alzheimer Disease
Wavelength
Monitoring

Keywords

  • β-amyloid
  • Alzheimer's disease
  • Blood
  • Fluorescence

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Whole blood samples from Alzheimer patients and control donors demonstrate fluorimetric differences. / Palotàs, Andràs; Kálmán, J.; Palotàs, Miklós; Matin, Keyvan; Szentpáli, Károly; Paszt, Attila; Janka, Z.; Penke, B.

In: Neurochemical Research, Vol. 27, No. 12, 01.12.2002, p. 1589-1591.

Research output: Contribution to journalArticle

Palotàs, Andràs ; Kálmán, J. ; Palotàs, Miklós ; Matin, Keyvan ; Szentpáli, Károly ; Paszt, Attila ; Janka, Z. ; Penke, B. / Whole blood samples from Alzheimer patients and control donors demonstrate fluorimetric differences. In: Neurochemical Research. 2002 ; Vol. 27, No. 12. pp. 1589-1591.
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