Which parameters affect cytosolic free calcium in polymorphonuclear leukocytes of haemodialysis patients?

István Kárpáti, I. Seres, J. Mátyus, Thomas Ben, G. Paragh, Z. Varga, G. Kakuk

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13 Citations (Scopus)

Abstract

Background. Cytosolic free calcium ([Ca2+]i) is an important second messenger during stimulation in a wide variety of cells, including polymorphonuclear leukocytes (PMNs). Its mobilization in PMNs is altered in various diseases such as atherosclerosis and ageing. In chronic haemodialysis (HD) patients, both atherosclerosis and accelerated ageing are well known. Therefore [Ca2+]i in resting PMNs of HD patients was determined along with certain parameters which might affect it, such as recombinant human erythropoietin (rHuEpo) treatment, calcium-phosphate balance, and biocompatibility of dialysis membranes. Methods. PMNs were separated by density centrifugation and [Ca2+]i was determined by spectrofluorimetry using Quin 2/AM fluorescent dye. Laboratory parameters were determined by standard methods in clinical chemistry. Results. It was found that [Ca2+]i in resting PMNs of HD patients not undergoing rHuEpo therapy was higher than that of controls. After 12-weeks of rHuEpo therapy, [Ca2+]i decreased to near normal level. The role of erythropoiesis in normalization of [Ca2+]i in resting PMNs was supported by PMN [Ca2+]i which was elevated in patients who had low haemoglobin (2+]i remained high, suggesting a role for other parameters in increasing [Ca2+]i. One possible parameter might be the disturbed calcium-phosphate metabolism of chronic renal failure, because we found a strong correlation between [Ca2+]i and plasma iPTH levels in HD patients (r=0.743, P2+]i was also elevated in PMNs of those patients who had either low plasma calcium or high plasma phosphate levels. PMN [Ca2+]i of HD patients correlated positively with the duration of HD (r=0.671, P2+]i and patient age. The dialysis procedure itself also transiently increased PMN [Ca2+]i HD patients, independently of the type of dialysis membrane. Conclusion. PMN [Ca2+]i is modulated by various parameters in HD patients, including the degree of anaemia, disturbances of calcium metabolism, and duration of dialysis treatment. The elevated [Ca2+]i of resting PMNs might contribute to altered functions in these cells.

Original languageEnglish
Pages (from-to)1409-1415
Number of pages7
JournalNephrology Dialysis Transplantation
Volume16
Issue number7
Publication statusPublished - 2001

Fingerprint

Renal Dialysis
Neutrophils
Calcium
Dialysis
Erythropoietin
Atherosclerosis
Clinical Chemistry
Membranes
Erythropoiesis
Second Messenger Systems
Therapeutics
Centrifugation
Fluorescent Dyes
Chronic Kidney Failure
Anemia
Hemoglobins
Phosphates

Keywords

  • Cytosolic free calcium
  • Erythropoietin
  • Haemodialysis
  • iPTH
  • Polymorphonuclear leukocytes

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

@article{4bdab21907c04646bd1087282c77aacd,
title = "Which parameters affect cytosolic free calcium in polymorphonuclear leukocytes of haemodialysis patients?",
abstract = "Background. Cytosolic free calcium ([Ca2+]i) is an important second messenger during stimulation in a wide variety of cells, including polymorphonuclear leukocytes (PMNs). Its mobilization in PMNs is altered in various diseases such as atherosclerosis and ageing. In chronic haemodialysis (HD) patients, both atherosclerosis and accelerated ageing are well known. Therefore [Ca2+]i in resting PMNs of HD patients was determined along with certain parameters which might affect it, such as recombinant human erythropoietin (rHuEpo) treatment, calcium-phosphate balance, and biocompatibility of dialysis membranes. Methods. PMNs were separated by density centrifugation and [Ca2+]i was determined by spectrofluorimetry using Quin 2/AM fluorescent dye. Laboratory parameters were determined by standard methods in clinical chemistry. Results. It was found that [Ca2+]i in resting PMNs of HD patients not undergoing rHuEpo therapy was higher than that of controls. After 12-weeks of rHuEpo therapy, [Ca2+]i decreased to near normal level. The role of erythropoiesis in normalization of [Ca2+]i in resting PMNs was supported by PMN [Ca2+]i which was elevated in patients who had low haemoglobin (2+]i remained high, suggesting a role for other parameters in increasing [Ca2+]i. One possible parameter might be the disturbed calcium-phosphate metabolism of chronic renal failure, because we found a strong correlation between [Ca2+]i and plasma iPTH levels in HD patients (r=0.743, P2+]i was also elevated in PMNs of those patients who had either low plasma calcium or high plasma phosphate levels. PMN [Ca2+]i of HD patients correlated positively with the duration of HD (r=0.671, P2+]i and patient age. The dialysis procedure itself also transiently increased PMN [Ca2+]i HD patients, independently of the type of dialysis membrane. Conclusion. PMN [Ca2+]i is modulated by various parameters in HD patients, including the degree of anaemia, disturbances of calcium metabolism, and duration of dialysis treatment. The elevated [Ca2+]i of resting PMNs might contribute to altered functions in these cells.",
keywords = "Cytosolic free calcium, Erythropoietin, Haemodialysis, iPTH, Polymorphonuclear leukocytes",
author = "Istv{\'a}n K{\'a}rp{\'a}ti and I. Seres and J. M{\'a}tyus and Thomas Ben and G. Paragh and Z. Varga and G. Kakuk",
year = "2001",
language = "English",
volume = "16",
pages = "1409--1415",
journal = "Nephrology Dialysis Transplantation",
issn = "0931-0509",
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TY - JOUR

T1 - Which parameters affect cytosolic free calcium in polymorphonuclear leukocytes of haemodialysis patients?

AU - Kárpáti, István

AU - Seres, I.

AU - Mátyus, J.

AU - Ben, Thomas

AU - Paragh, G.

AU - Varga, Z.

AU - Kakuk, G.

PY - 2001

Y1 - 2001

N2 - Background. Cytosolic free calcium ([Ca2+]i) is an important second messenger during stimulation in a wide variety of cells, including polymorphonuclear leukocytes (PMNs). Its mobilization in PMNs is altered in various diseases such as atherosclerosis and ageing. In chronic haemodialysis (HD) patients, both atherosclerosis and accelerated ageing are well known. Therefore [Ca2+]i in resting PMNs of HD patients was determined along with certain parameters which might affect it, such as recombinant human erythropoietin (rHuEpo) treatment, calcium-phosphate balance, and biocompatibility of dialysis membranes. Methods. PMNs were separated by density centrifugation and [Ca2+]i was determined by spectrofluorimetry using Quin 2/AM fluorescent dye. Laboratory parameters were determined by standard methods in clinical chemistry. Results. It was found that [Ca2+]i in resting PMNs of HD patients not undergoing rHuEpo therapy was higher than that of controls. After 12-weeks of rHuEpo therapy, [Ca2+]i decreased to near normal level. The role of erythropoiesis in normalization of [Ca2+]i in resting PMNs was supported by PMN [Ca2+]i which was elevated in patients who had low haemoglobin (2+]i remained high, suggesting a role for other parameters in increasing [Ca2+]i. One possible parameter might be the disturbed calcium-phosphate metabolism of chronic renal failure, because we found a strong correlation between [Ca2+]i and plasma iPTH levels in HD patients (r=0.743, P2+]i was also elevated in PMNs of those patients who had either low plasma calcium or high plasma phosphate levels. PMN [Ca2+]i of HD patients correlated positively with the duration of HD (r=0.671, P2+]i and patient age. The dialysis procedure itself also transiently increased PMN [Ca2+]i HD patients, independently of the type of dialysis membrane. Conclusion. PMN [Ca2+]i is modulated by various parameters in HD patients, including the degree of anaemia, disturbances of calcium metabolism, and duration of dialysis treatment. The elevated [Ca2+]i of resting PMNs might contribute to altered functions in these cells.

AB - Background. Cytosolic free calcium ([Ca2+]i) is an important second messenger during stimulation in a wide variety of cells, including polymorphonuclear leukocytes (PMNs). Its mobilization in PMNs is altered in various diseases such as atherosclerosis and ageing. In chronic haemodialysis (HD) patients, both atherosclerosis and accelerated ageing are well known. Therefore [Ca2+]i in resting PMNs of HD patients was determined along with certain parameters which might affect it, such as recombinant human erythropoietin (rHuEpo) treatment, calcium-phosphate balance, and biocompatibility of dialysis membranes. Methods. PMNs were separated by density centrifugation and [Ca2+]i was determined by spectrofluorimetry using Quin 2/AM fluorescent dye. Laboratory parameters were determined by standard methods in clinical chemistry. Results. It was found that [Ca2+]i in resting PMNs of HD patients not undergoing rHuEpo therapy was higher than that of controls. After 12-weeks of rHuEpo therapy, [Ca2+]i decreased to near normal level. The role of erythropoiesis in normalization of [Ca2+]i in resting PMNs was supported by PMN [Ca2+]i which was elevated in patients who had low haemoglobin (2+]i remained high, suggesting a role for other parameters in increasing [Ca2+]i. One possible parameter might be the disturbed calcium-phosphate metabolism of chronic renal failure, because we found a strong correlation between [Ca2+]i and plasma iPTH levels in HD patients (r=0.743, P2+]i was also elevated in PMNs of those patients who had either low plasma calcium or high plasma phosphate levels. PMN [Ca2+]i of HD patients correlated positively with the duration of HD (r=0.671, P2+]i and patient age. The dialysis procedure itself also transiently increased PMN [Ca2+]i HD patients, independently of the type of dialysis membrane. Conclusion. PMN [Ca2+]i is modulated by various parameters in HD patients, including the degree of anaemia, disturbances of calcium metabolism, and duration of dialysis treatment. The elevated [Ca2+]i of resting PMNs might contribute to altered functions in these cells.

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KW - Erythropoietin

KW - Haemodialysis

KW - iPTH

KW - Polymorphonuclear leukocytes

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