Wheat storage proteins in transgenic rice endosperm

Mária Oszvald, Gábor Balázs, Sára Pólya, Sándor Tömösközi, Rudi Appels, Ferenc Békés, László Tamás

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Transgenic rice seed expressing wheat HMW glutenin subunit was characterized to study the effects of the wheat prolamin on the protein expression pattern and protein size distribution in the endosperm and the functional and rheological properties of the rice flour and dough. Significant differences were found in the protein expression pattern between the transgenic and wild type samples. Comparing the protein expression profiles of transgenic and nontransgenic plants, combined with proteomic-based studies, indicated increased protein disulfide isomerase (PDI) levels in the transgenic rice lines. The accurate molecular size of HMW-GS in rice endosperm was identified by MALDI-TOF-MS analysis. The expressed wheat HMW (subunit 1Dx5) GS showed a positive effect on the functional properties of rice dough by significantly increasing the size distribution of the polymeric protein fraction and modifying the dough mixing parameters.

Original languageEnglish
Pages (from-to)7606-7614
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number31
DOIs
Publication statusPublished - Aug 7 2013

Keywords

  • proteomics
  • rice dough
  • transgenic rice
  • wheat glutenin subunit

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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  • Cite this

    Oszvald, M., Balázs, G., Pólya, S., Tömösközi, S., Appels, R., Békés, F., & Tamás, L. (2013). Wheat storage proteins in transgenic rice endosperm. Journal of Agricultural and Food Chemistry, 61(31), 7606-7614. https://doi.org/10.1021/jf402035n