What limits the velocity of fast-skeletal muscle contraction in mammals?

M. Nyitrai, Rosetta Rossi, Nancy Adamek, Maria Antonietta Pellegrino, Roberto Bottinelli, Michael A. Geeves

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

In rat skeletal muscle the unloaded shortening velocity (Vo) is defined by the myosin isoform expressed in the muscle fibre. In 2001 we suggested that ADP release from actomyosin in solution (controlled by k -AD) was of the right size to limit Vo. However, to compare mechanical and solution kinetic data required a series of corrections to compensate for the differences in experimental conditions (0.5 M KCl, 22°C for kinetic assays of myosin, 200 mM ionic strength, 12°C to measure V o). Here, a method was developed to prepare heavy meromyosin (HMM) from pure myosin isoforms isolated from single muscle fibres and to study k -AD (determined from the affinity of the acto-myosin complex for ADP, KAD) and the rate of ATP-induced acto-HMM dissociation (controlled by K1k+2) under the same experimental condition used to measure Vo. In fast-muscle myosin isolated from a wide range of mammalian muscles, k-AD was found to be too fast to limit V o, whereas K1k+2 was of the right magnitude for ATP-induced dissociation of the cross-bridge to limit shortening velocity. The result was unexpected and prompted further experiments using the stopped-flow approach on myosin subfragment-1 (S1) and HMM obtained from bulk preparations of rabbit and rat muscle. These confirmed that the rate of cross-bridge dissociation by ATP limits the velocity of contraction for fast myosin II isoforms at 12°C, while k-AD limits the velocity of slow myosin II isoforms. Extrapolating our data to 37°C suggests that at physiological temperature the rate of ADP dissociation may limit Vo for both isoforms.

Original languageEnglish
Pages (from-to)432-442
Number of pages11
JournalJournal of Molecular Biology
Volume355
Issue number3
DOIs
Publication statusPublished - Jan 20 2006

Fingerprint

Myosin Subfragments
Myosins
Muscle Contraction
pioglitazone
Mammals
Protein Isoforms
Skeletal Muscle
Muscles
Adenosine Diphosphate
Myosin Type II
Adenosine Triphosphate
Actomyosin
Osmolar Concentration
Rabbits
Temperature

Keywords

  • Fast kinetics
  • Flash photoplysis
  • Myosin isoforms
  • Subfragment 1
  • Temperature dependence

ASJC Scopus subject areas

  • Virology

Cite this

Nyitrai, M., Rossi, R., Adamek, N., Pellegrino, M. A., Bottinelli, R., & Geeves, M. A. (2006). What limits the velocity of fast-skeletal muscle contraction in mammals? Journal of Molecular Biology, 355(3), 432-442. https://doi.org/10.1016/j.jmb.2005.10.063

What limits the velocity of fast-skeletal muscle contraction in mammals? / Nyitrai, M.; Rossi, Rosetta; Adamek, Nancy; Pellegrino, Maria Antonietta; Bottinelli, Roberto; Geeves, Michael A.

In: Journal of Molecular Biology, Vol. 355, No. 3, 20.01.2006, p. 432-442.

Research output: Contribution to journalArticle

Nyitrai, M, Rossi, R, Adamek, N, Pellegrino, MA, Bottinelli, R & Geeves, MA 2006, 'What limits the velocity of fast-skeletal muscle contraction in mammals?', Journal of Molecular Biology, vol. 355, no. 3, pp. 432-442. https://doi.org/10.1016/j.jmb.2005.10.063
Nyitrai, M. ; Rossi, Rosetta ; Adamek, Nancy ; Pellegrino, Maria Antonietta ; Bottinelli, Roberto ; Geeves, Michael A. / What limits the velocity of fast-skeletal muscle contraction in mammals?. In: Journal of Molecular Biology. 2006 ; Vol. 355, No. 3. pp. 432-442.
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