WFIKKN1 and WFIKKN2 bind growth factors TGF β 1, BMP2 and BMP4 but do not inhibit their signalling activity

György Szláma, Katalin Kondás, M. Trexler, L. Patthy

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

WFIKKN1 and WFIKKN2 are large extracellular multidomain proteins consisting of a WAP domain, a follistatin domain, an immunoglobulin domain, two Kunitz-type protease inhibitor domains and an NTR domain. Recent experiments have shown that both proteins have high affinity for growth and differentiation factor (GDF)8 and GDF11. Here we study the interaction of WFIKKN proteins with several additional representatives of the transforming growth factor (TGF)β family using SPR measurements. Analyses of SPR sensorgrams suggested that, in addition to GDF8 and GDF11, both WFIKKN proteins bind TGFβ1, bone morphogenetic protein (BMP)2 and BMP4 with relatively high affinity (K d ∼ 10 -6 m). To assess the biological significance of these interactions we studied the effect of WFIKKN proteins on the activity of GDF8, GDF11, TGFβ1, BMP2 and BMP4 using reporter assays. These studies revealed that WFIKKN1 and WFIKKN2 inhibited the biological activity of GDF8 and GDF11 in the nanomolar range, whereas they did not inhibit the activities of TGFβ1, BMP2 and BMP4 even in the micromolar range. Our data indicate that WFIKKN proteins are antagonists of GDF8 and GDF11, but in the case of TGFβ1, BMP2 and BMP4 they function as growth factor binding proteins. It is suggested that the physical association of WFIKKN proteins with these growth factors may localize their action and thus help to establish growth factor gradients in the extracellular space.

Original languageEnglish
Pages (from-to)5040-5050
Number of pages11
JournalFEBS Journal
Volume277
Issue number24
DOIs
Publication statusPublished - Dec 2010

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Transforming Growth Factors
Intercellular Signaling Peptides and Proteins
Proteins
Myostatin
Follistatin
Bone Morphogenetic Protein 2
Extracellular Space
Bioactivity
Protease Inhibitors
Immunoglobulins
Assays
Carrier Proteins
Association reactions

Keywords

  • BMP
  • GDF11
  • GDF8
  • TGFβ
  • WFIKKN

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

WFIKKN1 and WFIKKN2 bind growth factors TGF β 1, BMP2 and BMP4 but do not inhibit their signalling activity. / Szláma, György; Kondás, Katalin; Trexler, M.; Patthy, L.

In: FEBS Journal, Vol. 277, No. 24, 12.2010, p. 5040-5050.

Research output: Contribution to journalArticle

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AU - Patthy, L.

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