Wavelength dependency of light-induced changes in rhythmic melatonin secretion from chicken pineal gland in vitro

Valér Csernus, Péter Becher, Béla Mess

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Avian pineals show rhythmic, circadian melatonin secretion pattern also in vitro. The phase of this rhythm can be modified by changing the illumination. Reversed in vitro illumination reverses the phase of the circadian melatonin rhythm in two days. In the present study the effect of the wavelength on the speed of the phase shift of the melatonin rhythm of the explanted chicken pineals was investigated in a dynamic in vitro bioassay. Chicken pineals were placed into perifusion columns and the tissue was exposed to reversed illumination through various light filters. Changes in the melatonin release during 4 day long experiments were studied. Clear differences in the speed of the reversal of the melatonin rhythm were observed as a function of the transmission spectrum of the light filters. The shorter the transmitted wavelength, the more rapid the phase shift was found. These data are in good agreement with earlier studies showing that the chicken pineal photo-pigment, pinopsin, is a blue light sensitive molecule. Our observation reveals that the blue light sensitive pigment is not only present but also fully functional, in controlling the circadian biological clock in the chicken pineal gland.

Original languageEnglish
Pages (from-to)299-304
Number of pages6
JournalNeuroendocrinology Letters
Volume20
Issue number5
Publication statusPublished - Jan 1 1999

Keywords

  • Chicken pineal
  • Circadian rhythm
  • In vitro
  • Light wavelength
  • Melatonin
  • Perifusion

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems
  • Clinical Neurology
  • Psychiatry and Mental health

Fingerprint Dive into the research topics of 'Wavelength dependency of light-induced changes in rhythmic melatonin secretion from chicken pineal gland in vitro'. Together they form a unique fingerprint.

  • Cite this