Volume regulation of chinese hamster ovary cells in anisoosmotic media

B. Sarkadi, L. Attisano, S. Grinstein, M. Buchwald, A. Rothstein

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Chinese hamster ovary (CHO) cells when suspended in anisoosmotic media regulate their volumes by the activation of specific ion transport pathways. In hypoosmotic media the cells first swell and then return to their isoosmotic volumes by the loss of cellular KCl and osmotically obliged water. This regulatory volume decrease (RVD) is insensitive to ouabain or bumetanide but is blocked by quinine, cetiedil and oligomycin C. Based on cell volume and membrane potential measurements under various experimental conditions, we conclude that hypoosmotic shock activates independent, conductive transport pathways for K+ and for Cl-, respectively. The anion pathway can also transport NO3- and SCN- but not gluconate- anions. Osmotic shrinkage of CHO cells does not produce a regulatory volume increase (RVI) unless the cells have previously undergone a cycle of RVD. RVI is a Na+-dependent, amiloride-sensitive, but ouabain- and oligomycin-insensitive process, probably involving a Na+-H+ exchange system. Internal acidification of isoosmotic cells by addition of a permeable weak acid also activates an amiloride-sensitive Na+-H+ exchange, producing a volume increase. Both RVD and RVI in CHO cells seem to involve molecular mechanisms similar to those described for the volume regulation of lymphocytes, indicating the prevalence of these phenomena in nucleated mammalian cells. Cultured CHO cell lines may provide a basis for a genetic characterization of the volume-regulatory transport pathways.

Original languageEnglish
Pages (from-to)159-168
Number of pages10
JournalBBA - Biomembranes
Volume774
Issue number2
DOIs
Publication statusPublished - Jul 25 1984

Fingerprint

Cricetulus
Ovary
Amiloride
Cells
Ouabain
Anions
Bumetanide
Oligomycins
Quinine
Lymphocytes
Acidification
Chemical activation
Ions
Membranes
Acids
Water
Ion Transport
Cell Size
Membrane Potentials
Shock

Keywords

  • (Chinese hamster ovary cell)
  • Hypoosmotic shock
  • Ion transport
  • Membrane potential
  • Volume regulation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology

Cite this

Volume regulation of chinese hamster ovary cells in anisoosmotic media. / Sarkadi, B.; Attisano, L.; Grinstein, S.; Buchwald, M.; Rothstein, A.

In: BBA - Biomembranes, Vol. 774, No. 2, 25.07.1984, p. 159-168.

Research output: Contribution to journalArticle

Sarkadi, B, Attisano, L, Grinstein, S, Buchwald, M & Rothstein, A 1984, 'Volume regulation of chinese hamster ovary cells in anisoosmotic media', BBA - Biomembranes, vol. 774, no. 2, pp. 159-168. https://doi.org/10.1016/0005-2736(84)90287-6
Sarkadi, B. ; Attisano, L. ; Grinstein, S. ; Buchwald, M. ; Rothstein, A. / Volume regulation of chinese hamster ovary cells in anisoosmotic media. In: BBA - Biomembranes. 1984 ; Vol. 774, No. 2. pp. 159-168.
@article{b74300a1b5ea423994eae03f1b0f2ec2,
title = "Volume regulation of chinese hamster ovary cells in anisoosmotic media",
abstract = "Chinese hamster ovary (CHO) cells when suspended in anisoosmotic media regulate their volumes by the activation of specific ion transport pathways. In hypoosmotic media the cells first swell and then return to their isoosmotic volumes by the loss of cellular KCl and osmotically obliged water. This regulatory volume decrease (RVD) is insensitive to ouabain or bumetanide but is blocked by quinine, cetiedil and oligomycin C. Based on cell volume and membrane potential measurements under various experimental conditions, we conclude that hypoosmotic shock activates independent, conductive transport pathways for K+ and for Cl-, respectively. The anion pathway can also transport NO3- and SCN- but not gluconate- anions. Osmotic shrinkage of CHO cells does not produce a regulatory volume increase (RVI) unless the cells have previously undergone a cycle of RVD. RVI is a Na+-dependent, amiloride-sensitive, but ouabain- and oligomycin-insensitive process, probably involving a Na+-H+ exchange system. Internal acidification of isoosmotic cells by addition of a permeable weak acid also activates an amiloride-sensitive Na+-H+ exchange, producing a volume increase. Both RVD and RVI in CHO cells seem to involve molecular mechanisms similar to those described for the volume regulation of lymphocytes, indicating the prevalence of these phenomena in nucleated mammalian cells. Cultured CHO cell lines may provide a basis for a genetic characterization of the volume-regulatory transport pathways.",
keywords = "(Chinese hamster ovary cell), Hypoosmotic shock, Ion transport, Membrane potential, Volume regulation",
author = "B. Sarkadi and L. Attisano and S. Grinstein and M. Buchwald and A. Rothstein",
year = "1984",
month = "7",
day = "25",
doi = "10.1016/0005-2736(84)90287-6",
language = "English",
volume = "774",
pages = "159--168",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Volume regulation of chinese hamster ovary cells in anisoosmotic media

AU - Sarkadi, B.

AU - Attisano, L.

AU - Grinstein, S.

AU - Buchwald, M.

AU - Rothstein, A.

PY - 1984/7/25

Y1 - 1984/7/25

N2 - Chinese hamster ovary (CHO) cells when suspended in anisoosmotic media regulate their volumes by the activation of specific ion transport pathways. In hypoosmotic media the cells first swell and then return to their isoosmotic volumes by the loss of cellular KCl and osmotically obliged water. This regulatory volume decrease (RVD) is insensitive to ouabain or bumetanide but is blocked by quinine, cetiedil and oligomycin C. Based on cell volume and membrane potential measurements under various experimental conditions, we conclude that hypoosmotic shock activates independent, conductive transport pathways for K+ and for Cl-, respectively. The anion pathway can also transport NO3- and SCN- but not gluconate- anions. Osmotic shrinkage of CHO cells does not produce a regulatory volume increase (RVI) unless the cells have previously undergone a cycle of RVD. RVI is a Na+-dependent, amiloride-sensitive, but ouabain- and oligomycin-insensitive process, probably involving a Na+-H+ exchange system. Internal acidification of isoosmotic cells by addition of a permeable weak acid also activates an amiloride-sensitive Na+-H+ exchange, producing a volume increase. Both RVD and RVI in CHO cells seem to involve molecular mechanisms similar to those described for the volume regulation of lymphocytes, indicating the prevalence of these phenomena in nucleated mammalian cells. Cultured CHO cell lines may provide a basis for a genetic characterization of the volume-regulatory transport pathways.

AB - Chinese hamster ovary (CHO) cells when suspended in anisoosmotic media regulate their volumes by the activation of specific ion transport pathways. In hypoosmotic media the cells first swell and then return to their isoosmotic volumes by the loss of cellular KCl and osmotically obliged water. This regulatory volume decrease (RVD) is insensitive to ouabain or bumetanide but is blocked by quinine, cetiedil and oligomycin C. Based on cell volume and membrane potential measurements under various experimental conditions, we conclude that hypoosmotic shock activates independent, conductive transport pathways for K+ and for Cl-, respectively. The anion pathway can also transport NO3- and SCN- but not gluconate- anions. Osmotic shrinkage of CHO cells does not produce a regulatory volume increase (RVI) unless the cells have previously undergone a cycle of RVD. RVI is a Na+-dependent, amiloride-sensitive, but ouabain- and oligomycin-insensitive process, probably involving a Na+-H+ exchange system. Internal acidification of isoosmotic cells by addition of a permeable weak acid also activates an amiloride-sensitive Na+-H+ exchange, producing a volume increase. Both RVD and RVI in CHO cells seem to involve molecular mechanisms similar to those described for the volume regulation of lymphocytes, indicating the prevalence of these phenomena in nucleated mammalian cells. Cultured CHO cell lines may provide a basis for a genetic characterization of the volume-regulatory transport pathways.

KW - (Chinese hamster ovary cell)

KW - Hypoosmotic shock

KW - Ion transport

KW - Membrane potential

KW - Volume regulation

UR - http://www.scopus.com/inward/record.url?scp=0021140709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021140709&partnerID=8YFLogxK

U2 - 10.1016/0005-2736(84)90287-6

DO - 10.1016/0005-2736(84)90287-6

M3 - Article

VL - 774

SP - 159

EP - 168

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 2

ER -