Voltage gated calcium channel activation by backpropagating action potentials downregulates NMDAR function

Anne Kathrin Theis, B. Rózsa, Gergely Katona, Dietmar Schmitz, Friedrich W. Johenning

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The majority of excitatory synapses are located on dendritic spines of cortical glutamatergic neurons. In spines, compartmentalized Ca2+ signals transduce electrical activity into specific long-term biochemical and structural changes. Action potentials (APs) propagate back into the dendritic tree and activate voltage gated Ca2+ channels (VGCCs). For spines, this global mode of spine Ca2+ signaling is a direct biochemical feedback of suprathreshold neuronal activity. We previously demonstrated that backpropagating action potentials (bAPs) result in long-term enhancement of spine VGCCs. This activity-dependent VGCC plasticity results in a large interspine variability of VGCC Ca2+ influx. Here, we investigate how spine VGCCs affect glutamatergic synaptic transmission. We combined electrophysiology, two-photon Ca2+ imaging and two-photon glutamate uncaging in acute brain slices from rats. T- and R-type VGCCs were the dominant depolarization-associated Ca2+ conductances in dendritic spines of excitatory layer 2 neurons and do not affect synaptic excitatory postsynaptic potentials (EPSPs) measured at the soma. Using two-photon glutamate uncaging, we compared the properties of glutamatergic synapses of single spines that express different levels of VGCCs. While VGCCs contributed to EPSP mediated Ca2+ influx, the amount of EPSP mediated Ca2+ influx is not determined by spine VGCC expression. On a longer timescale, the activation of VGCCs by bAP bursts results in downregulation of spine NMDAR function.

Original languageEnglish
Article number109
JournalFrontiers in Cellular Neuroscience
Volume12
DOIs
Publication statusPublished - Apr 23 2018

Fingerprint

Calcium Channels
Action Potentials
Spine
Down-Regulation
Excitatory Postsynaptic Potentials
Photons
Dendritic Spines
Synapses
Glutamic Acid
Physiological Feedback
Neurons
Electrophysiology
Carisoprodol
Synaptic Transmission
Brain

Keywords

  • Calcium
  • Dendritic spines
  • Homeostatic synaptic plasticity
  • Metaplasticity
  • NMDAR
  • Synaptic transmission
  • Two-photon microscopy
  • Voltage gated Ca channels (VGCCs)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Voltage gated calcium channel activation by backpropagating action potentials downregulates NMDAR function. / Theis, Anne Kathrin; Rózsa, B.; Katona, Gergely; Schmitz, Dietmar; Johenning, Friedrich W.

In: Frontiers in Cellular Neuroscience, Vol. 12, 109, 23.04.2018.

Research output: Contribution to journalArticle

Theis, Anne Kathrin ; Rózsa, B. ; Katona, Gergely ; Schmitz, Dietmar ; Johenning, Friedrich W. / Voltage gated calcium channel activation by backpropagating action potentials downregulates NMDAR function. In: Frontiers in Cellular Neuroscience. 2018 ; Vol. 12.
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