Voltage dependent calcium channels in adrenal glomerulosa cells and in insulin producing cells

A. Horváth, Gy Szabadkai, P. Várnai, T. Arányi, C. B. Wollheim, A. Spät, P. Enyedi

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Abstract

We have examined the structure and function of Ca2+ channels in excitable endocrine cell types, in rat adrenal glomerulosa cells and in two insulin producing cell types, the rat pancreatic β cell and the INS-1 cell line. In previous studies on glomerulosa cells, we observed low (T-type) and high threshold (L-type) voltage dependent Ca2+ currents in addition to a K+ induced inward rectifying Ca2+ current (I(gl)). β cells are known to exhibit T-, L- and N-type currents. We have now found that INS-1 cells also show low threshold (T-type) and high threshold Ca2+ currents. The latter was further resolved by organic inhibitors into L-type and P/Q-type currents and no I(gl) was detected. The expression of the pore-forming α1 subunit of voltage dependent Ca2+ channels was studied by means of reverse transcription-polymerase chain reaction (RT-PCR), followed by restriction enzyme mapping and/or sequencing. Both in glomerulosa and pancreatic β cells, the neuroendocrine (D) class of the α1 subunit, known to be responsible for L-type current, represents the majority of the PCR product. Comparable amounts of the neuroendocrine (D) and the neuronal A-type α1 subunits dominate the message in INS-1 cells. Different characteristics of Ca2+ currents in these cell types is discussed in view of the channel repertoire.

Original languageEnglish
Pages (from-to)33-42
Number of pages10
JournalCell Calcium
Volume23
Issue number1
DOIs
Publication statusPublished - Jan 1998

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Zona Glomerulosa
Calcium Channels
Insulin
Polymerase Chain Reaction
Restriction Mapping
Endocrine Cells
Somatostatin-Secreting Cells
Reverse Transcription
Cell Line

ASJC Scopus subject areas

  • Cell Biology
  • Endocrinology

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Voltage dependent calcium channels in adrenal glomerulosa cells and in insulin producing cells. / Horváth, A.; Szabadkai, Gy; Várnai, P.; Arányi, T.; Wollheim, C. B.; Spät, A.; Enyedi, P.

In: Cell Calcium, Vol. 23, No. 1, 01.1998, p. 33-42.

Research output: Contribution to journalArticle

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