VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation

Sarada D. Tetali, Madhu S. Budamagunta, Catalina Simion, Laura J. Den Hartigh, T. Kalai, K. Hideg, Danny M. Hatters, Karl H. Weisgraber, John C. Voss, John C. Rutledge

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Our previous work indicated that apolipoprotein (apo) E4 assumes a more expanded conformation in the postprandial period. The postprandial state is characterized by increased VLDL lipolysis. In this article, we tested the hypothesis that VLDL lipolysis products increase VLDL particle fluidity, which mediates expansion of apoE4 on the VLDL particle. Plasma from healthy subjects was collected before and after a moderately high-fat meal and incubated with nitroxyl-spin labeled apoE. ApoE conformation was examined by electron paramagnetic resonance spectroscopy using targeted spin probes on cysteines introduced in the N-terminal (S76C) and C-terminal (A241C) domains. Further, we synthesized a novel nitroxyl spin-labeled cholesterol analog, which gave insight into lipoprotein particle fluidity. Our data revealed that the order of lipoprotein fluidity was HDL∼LDL

Original languageEnglish
Pages (from-to)1273-1283
Number of pages11
JournalJournal of Lipid Research
Volume51
Issue number6
DOIs
Publication statusPublished - Jun 1 2010

Fingerprint

Apolipoprotein E4
Fluidity
Lipolysis
Apolipoproteins E
Lipoproteins
Conformations
Postprandial Period
Electron Spin Resonance Spectroscopy
Cysteine
Meals
Spectrum Analysis
Healthy Volunteers
Fats
Cholesterol
Paramagnetic resonance
Spectroscopy
Plasmas
nitroxyl

Keywords

  • Lipid fluidity
  • Postprandial state
  • Structural conformation
  • Very low density lipoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation. / Tetali, Sarada D.; Budamagunta, Madhu S.; Simion, Catalina; Den Hartigh, Laura J.; Kalai, T.; Hideg, K.; Hatters, Danny M.; Weisgraber, Karl H.; Voss, John C.; Rutledge, John C.

In: Journal of Lipid Research, Vol. 51, No. 6, 01.06.2010, p. 1273-1283.

Research output: Contribution to journalArticle

Tetali, SD, Budamagunta, MS, Simion, C, Den Hartigh, LJ, Kalai, T, Hideg, K, Hatters, DM, Weisgraber, KH, Voss, JC & Rutledge, JC 2010, 'VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation', Journal of Lipid Research, vol. 51, no. 6, pp. 1273-1283. https://doi.org/10.1194/jlr.M000406
Tetali, Sarada D. ; Budamagunta, Madhu S. ; Simion, Catalina ; Den Hartigh, Laura J. ; Kalai, T. ; Hideg, K. ; Hatters, Danny M. ; Weisgraber, Karl H. ; Voss, John C. ; Rutledge, John C. / VLDL lipolysis products increase VLDL fluidity and convert apolipoprotein E4 into a more expanded conformation. In: Journal of Lipid Research. 2010 ; Vol. 51, No. 6. pp. 1273-1283.
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