Vitrification of mouse embryos in two cryoprotectant solutions

S. Cseh, W. Horlacher, G. Brem, J. Corselli, J. Seregi, L. Solti, L. Bailey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The objective of this study was to compare the efficiency of 2 media on the vitrification of mouse compacted morulae, early blastocysts and expanded blastocysts after equilibration at room temperature or 4°C. Embryos were equilibrated for 10 min in either 25% VS3 (Rall Equilibration Medium, REM) or 10% glycerol + 20% propylene glycol (Massip Equilibration Medium, MEM) in DPBS at 20°C or 4°C. For vitrification either 100% VS3 (Rall Vitrification Medium, RVM) or 25% glycerol + 25% propylene glycol (Massip Vitrification Medium, MVM) in DPBS was used. Embryos equilibrated at room temperature were loaded in 20 μL of vitrification media into 250 μL straws and then immediately (30 sec) plunged into liquid nitrogen (LN2). After equilibration at 4°C the embryos were put into straws with 20 μL of precooled vitrification medium, and after 20 min at 4 °C they were plunged into LN2. Embryos from both groups were thawed in a 20 °C water bath for 20 sec, transferred to 1.0 M sucrose in DPBS for 5 min and then cultured for 24 to 48 h in Whitten's medium at 37 °C in 5% CO2 in air. In the groups of embryos prepared for vitrification at room temperature the survival rate of compact morulae vitrified in RVM was higher than those vitrified in MVM (65/70, 93% vs 49/74, 66%; P2 is not required. A 10-fold increase in the survival rate of expanded blastocysts can be achieved using low temperature equilibration (4°C) and MVM.

Original languageEnglish
Pages (from-to)103-113
Number of pages11
JournalTheriogenology
Volume52
Issue number1
DOIs
Publication statusPublished - Jul 1 1999

Fingerprint

Vitrification
vitrification
cryoprotectants
embryo (animal)
Embryonic Structures
mice
Blastocyst
blastocyst
Morula
ambient temperature
Propylene Glycol
Temperature
propylene glycol
morula
Glycerol
glycerol
straw
survival rate
Baths
Sucrose

Keywords

  • Cryopreservation
  • Embryo freezing
  • Equilibration
  • Mouse embryo
  • Vitrification

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Cseh, S., Horlacher, W., Brem, G., Corselli, J., Seregi, J., Solti, L., & Bailey, L. (1999). Vitrification of mouse embryos in two cryoprotectant solutions. Theriogenology, 52(1), 103-113. https://doi.org/10.1016/S0093-691X(99)00113-2

Vitrification of mouse embryos in two cryoprotectant solutions. / Cseh, S.; Horlacher, W.; Brem, G.; Corselli, J.; Seregi, J.; Solti, L.; Bailey, L.

In: Theriogenology, Vol. 52, No. 1, 01.07.1999, p. 103-113.

Research output: Contribution to journalArticle

Cseh, S, Horlacher, W, Brem, G, Corselli, J, Seregi, J, Solti, L & Bailey, L 1999, 'Vitrification of mouse embryos in two cryoprotectant solutions', Theriogenology, vol. 52, no. 1, pp. 103-113. https://doi.org/10.1016/S0093-691X(99)00113-2
Cseh, S. ; Horlacher, W. ; Brem, G. ; Corselli, J. ; Seregi, J. ; Solti, L. ; Bailey, L. / Vitrification of mouse embryos in two cryoprotectant solutions. In: Theriogenology. 1999 ; Vol. 52, No. 1. pp. 103-113.
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