Vitrification of biopsied mouse embryos

B. Baranyai, Sz Bodó, A. Dinnyés, Elen Gócza

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35% EG + 5% PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vitro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalActa Veterinaria Hungarica
Volume53
Issue number1
DOIs
Publication statusPublished - 2005

Fingerprint

Vitrification
vitrification
Morula
embryo (animal)
Cryopreservation
morula
Embryonic Structures
cryopreservation
Blastocyst
mice
blastocyst
straw
birth rate
Birth Rate
Term Birth
Blastomeres
Trehalose
blastomeres
Herpes Zoster
trehalose

Keywords

  • Cryopreservation
  • Embryo biopsy
  • Mouse
  • Solid surface vitrification

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Vitrification of biopsied mouse embryos. / Baranyai, B.; Bodó, Sz; Dinnyés, A.; Gócza, Elen.

In: Acta Veterinaria Hungarica, Vol. 53, No. 1, 2005, p. 103-112.

Research output: Contribution to journalArticle

Baranyai, B. ; Bodó, Sz ; Dinnyés, A. ; Gócza, Elen. / Vitrification of biopsied mouse embryos. In: Acta Veterinaria Hungarica. 2005 ; Vol. 53, No. 1. pp. 103-112.
@article{9bb589d37dbf4259b73b6bb35bfcd3e3,
title = "Vitrification of biopsied mouse embryos",
abstract = "Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35{\%} EG + 5{\%} PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vitro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.",
keywords = "Cryopreservation, Embryo biopsy, Mouse, Solid surface vitrification",
author = "B. Baranyai and Sz Bod{\'o} and A. Dinny{\'e}s and Elen G{\'o}cza",
year = "2005",
doi = "10.1556/AVet.53.2005.1.10",
language = "English",
volume = "53",
pages = "103--112",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Vitrification of biopsied mouse embryos

AU - Baranyai, B.

AU - Bodó, Sz

AU - Dinnyés, A.

AU - Gócza, Elen

PY - 2005

Y1 - 2005

N2 - Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35% EG + 5% PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vitro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.

AB - Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35% EG + 5% PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vitro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.

KW - Cryopreservation

KW - Embryo biopsy

KW - Mouse

KW - Solid surface vitrification

UR - http://www.scopus.com/inward/record.url?scp=14644444437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14644444437&partnerID=8YFLogxK

U2 - 10.1556/AVet.53.2005.1.10

DO - 10.1556/AVet.53.2005.1.10

M3 - Article

VL - 53

SP - 103

EP - 112

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 1

ER -