Vitrification of biopsied mouse embryos

B. Baranyai, Sz Bodó, A. Dinnyés, Elen Gócza

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35% EG + 5% PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vitro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalActa veterinaria Hungarica
Volume53
Issue number1
DOIs
Publication statusPublished - Mar 15 2005

Keywords

  • Cryopreservation
  • Embryo biopsy
  • Mouse
  • Solid surface vitrification

ASJC Scopus subject areas

  • veterinary(all)

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