Vitellogenin-6 is a major carbonylated protein in aged nematode, Caenorhabditis elegans

Akihiro Nakamura, Kayo Yasuda, Hirosi Adachi, Yoshihiko Sakurai, Naoaki Ishii, S. Goto

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Recently we found that protein carbonyl content increases with age in wild-type as well as long- and short-lived Caenorhabditis elegans nematodes in inverse correlation with life span. In the present study, we investigated carbonyl modification of individual proteins in young and old wild-type nematodes by two-dimensional immunoblot using antibodies against 2,4-dinitrophenylhydrazones. A protein with apparent molecular weight of 110 kDa was found to be a major carbonylated protein in aged animals. Amino acid sequence of peptide fragments of the protein was identical to that of vitellogenin-6, a yolk protein synthesized in and secreted from the intestine during egg-laying stage. Although the function(s) of the protein in aged nematodes is unclear, we suggest that the protein may have a role to protect other cellular components from oxidation because of its metal binding capacity.

Original languageEnglish
Pages (from-to)580-583
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number2
DOIs
Publication statusPublished - Oct 22 1999

Fingerprint

Vitellogenins
Caenorhabditis elegans
Proteins
Egg Proteins
Peptide Fragments
Intestines
Ovum
Amino Acid Sequence
Animals
Molecular Weight
Metals
Molecular weight
Amino Acids
Oxidation
Antibodies

Keywords

  • Aging
  • Nematode
  • Protein carbonyl
  • Vitellogenin-6

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Vitellogenin-6 is a major carbonylated protein in aged nematode, Caenorhabditis elegans. / Nakamura, Akihiro; Yasuda, Kayo; Adachi, Hirosi; Sakurai, Yoshihiko; Ishii, Naoaki; Goto, S.

In: Biochemical and Biophysical Research Communications, Vol. 264, No. 2, 22.10.1999, p. 580-583.

Research output: Contribution to journalArticle

Nakamura, Akihiro ; Yasuda, Kayo ; Adachi, Hirosi ; Sakurai, Yoshihiko ; Ishii, Naoaki ; Goto, S. / Vitellogenin-6 is a major carbonylated protein in aged nematode, Caenorhabditis elegans. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 264, No. 2. pp. 580-583.
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