Vitamin D deficiency causes inward hypertrophic remodeling and alters vascular reactivity of rat cerebral arterioles

Éva Pál, Leila Hadjadj, Zoltán Fontányi, Anna Monori-Kiss, Zsuzsanna Mezei, Norbert Lippai, A. Magyar, Andrea Heinzlmann, Gellért Karvaly, E. Monos, G. Nádasy, Z. Benyó, S. Várbíró

Research output: Contribution to journalArticle

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Abstract

Background and purpose Vitamin D deficiency (VDD) is a global health problem, which can lead to several pathophysiological consequences including cardiovascular diseases. Its impact on the cerebrovascular system is not well understood. The goal of the present work was to examine the effects of VDD on the morphological, biomechanical and functional properties of cerebral arterioles. Methods Four-week-old male Wistar rats (n = 11 per group) were either fed with vitamin D deficient diet or received conventional rat chow with per os vitamin D supplementation. Cardiovascular parameters and hormone levels (testosterone, androstenedione, progesterone and 25-hydroxyvitamin D) were measured during the study. After 8 weeks of treatment anterior cerebral artery segments were prepared and their morphological, biomechanical and functional properties were examined using pressure microangiometry. Resorcin-fuchsin and smooth muscle actin staining were used to detect elastic fiber density and smooth muscle cell counts in the vessel wall, respectively. Sections were immunostained for eNOS and COX-2 as well. Results VDD markedly increased the wall thickness, the wall-to-lumen ratio and the wall cross-sectional area of arterioles as well as the number of smooth muscle cells in the tunica media. As a consequence, tangential wall stress was significantly lower in the VDD group. In addition, VDD increased the myogenic as well as the uridine 5’-triphosphate-induced tone and impaired bradykinin-induced relaxation. Decreased eNOS and increased COX-2 expression were also observed in the endothelium of VDD animals. Conclusions VDD causes inward hypertrophic remodeling due to vascular smooth muscle cell proliferation and enhances the vessel tone probably because of increased vasoconstrictor prostanoid levels in young adult rats. In addition, the decreased eNOS expression results in endothelial dysfunction. These morphological and functional alterations can potentially compromise the cerebral circulation and lead to cerebrovascular disorders in VDD.

Original languageEnglish
Article numbere0192480
JournalPLoS One
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

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vitamin D deficiency
Vitamin D Deficiency
Arterioles
blood vessels
Vitamin D
Rats
rats
smooth muscle
Muscle
myocytes
Smooth Muscle Myocytes
prostaglandin synthase
vitamin D
functional properties
Cerebrovascular Circulation
cerebrovascular disorders
Rosaniline Dyes
Tunica Media
Anterior Cerebral Artery
Cerebrovascular Disorders

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Vitamin D deficiency causes inward hypertrophic remodeling and alters vascular reactivity of rat cerebral arterioles. / Pál, Éva; Hadjadj, Leila; Fontányi, Zoltán; Monori-Kiss, Anna; Mezei, Zsuzsanna; Lippai, Norbert; Magyar, A.; Heinzlmann, Andrea; Karvaly, Gellért; Monos, E.; Nádasy, G.; Benyó, Z.; Várbíró, S.

In: PLoS One, Vol. 13, No. 2, e0192480, 01.02.2018.

Research output: Contribution to journalArticle

Pál, Éva ; Hadjadj, Leila ; Fontányi, Zoltán ; Monori-Kiss, Anna ; Mezei, Zsuzsanna ; Lippai, Norbert ; Magyar, A. ; Heinzlmann, Andrea ; Karvaly, Gellért ; Monos, E. ; Nádasy, G. ; Benyó, Z. ; Várbíró, S. / Vitamin D deficiency causes inward hypertrophic remodeling and alters vascular reactivity of rat cerebral arterioles. In: PLoS One. 2018 ; Vol. 13, No. 2.
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AU - Hadjadj, Leila

AU - Fontányi, Zoltán

AU - Monori-Kiss, Anna

AU - Mezei, Zsuzsanna

AU - Lippai, Norbert

AU - Magyar, A.

AU - Heinzlmann, Andrea

AU - Karvaly, Gellért

AU - Monos, E.

AU - Nádasy, G.

AU - Benyó, Z.

AU - Várbíró, S.

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