Visualization of non-synaptic release sites in the myenteric plexus of the snail Helix pomatia

I. Benedeczky, K. Halasy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Following conventional glutaraldehyde osmium tetroxidc fixation, a rich mycnteric plexus was detected in the gastrointestinal tract of the snail Helix pomatia. Although hundreds of nerve processes were observed in the extensive myoneural neuropil, true synaptic specializations were not recognized in them. In the absence of synaptic specializations, tannic acid Ringer incubation was applied to visualize the non-synaptic release sites in the enteric nerve plexus. After incubation for I h. a great number of exocytosis profiles were recorded at nerve muscle contacts, at axoglial connections and in the myoneural neuropil. The frequency of occurrence of exocytosis profiles was the same for adrenergic and peptidergic libres. In some gut wall areas, a dense staining of both basal lamina and collagen Ohres was observed. Invagination of dense basal lamina into the omega-shaped profiles of the axolemma led to "false exocytosis" profiles. A detailed morphological analysis is needed to distinguish false exocytosis profiles from the true transmitter-releasing loci.

Original languageEnglish
Pages (from-to)163-170
Number of pages8
JournalNeuroscience
Volume25
Issue number1
DOIs
Publication statusPublished - 1988

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Helix (Snails)
Myenteric Plexus
Exocytosis
Neuropil
Basement Membrane
Osmium
Tannins
Glutaral
Adrenergic Agents
Gastrointestinal Tract
Collagen
Staining and Labeling
Muscles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Visualization of non-synaptic release sites in the myenteric plexus of the snail Helix pomatia. / Benedeczky, I.; Halasy, K.

In: Neuroscience, Vol. 25, No. 1, 1988, p. 163-170.

Research output: Contribution to journalArticle

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