Visualization of calcium dynamics in kidney proximal tubules

Kornélia Szebényi, András Füredi, Orsolya Kolacsek, Rózsa Csohány, Ágnes Prókai, Katalin Kis-Petik, Attila Szabó, Zsuzsanna Bosze, Balázs Bender, József Tóvári, Ágnes Enyedi, Tamás I. Orbán, Ágota Apáti, Balázs Sarkadi

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17 Citations (Scopus)

Abstract

Intrarenal changes in cytoplasmic calciumlevels have a key role in determining pathologic and pharmacologic responses inmajor kidney diseases. However, cell-specific delivery of calcium-sensitive probes in vivo remains problematic. We generated a transgenic rat stably expressing the green fluorescent protein-calmodulin- based genetically encoded calcium indicator (GCaMP2) predominantly in the kidney proximal tubules. The transposon-based method used allowed the generation of homozygous transgenic rats containing one copy of the transgene per allele with a defined insertion pattern, without genetic or phenotypic alterations. We applied in vitro confocal and in vivo two-photon microscopy to examine basal calcium levels and ligand- and drug-induced alterations in these levels in proximal tubular epithelial cells. Notably, renal ischemia induced a transient increase in cellular calcium, and reperfusion resulted in a secondary calcium load, which was significantly decreased by systemic administration of specific blockers of the angiotensin receptor and the Na-Ca exchanger. The parallel examination of in vivo cellular calcium dynamics and renal circulation by fluorescent probes opens new possibilities for physiologic and pharmacologic investigations.

Original languageEnglish
Pages (from-to)2731-2740
Number of pages10
JournalJournal of the American Society of Nephrology
Volume26
Issue number11
DOIs
Publication statusPublished - Nov 2015

ASJC Scopus subject areas

  • Nephrology

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    Szebényi, K., Füredi, A., Kolacsek, O., Csohány, R., Prókai, Á., Kis-Petik, K., Szabó, A., Bosze, Z., Bender, B., Tóvári, J., Enyedi, Á., Orbán, T. I., Apáti, Á., & Sarkadi, B. (2015). Visualization of calcium dynamics in kidney proximal tubules. Journal of the American Society of Nephrology, 26(11), 2731-2740. https://doi.org/10.1681/ASN.2014070705