Visualization and characterization of prolamellar bodies with atomic force microscopy

Joanna M. Grzyb, K. Solymosi, Kazimierz Strzałka, Beata Mysliwa-Kurdziel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prolamellar bodies (PLBs) isolated from etiolated wheat seedlings were studied with the use of atomic force microscopy (AFM), transmission electron microscopy (TEM) and fluorescence spectroscopy. With AFM, PLBs were seen as spherical structures about 1-2μm in diameter, more elastic than mica and poly-l-lysine substrate. TEM analyses confirmed that PLBs of wheat leaf etioplasts also had an average diameter of appr. 1μm. Illumination induced the photoreduction of photoactive protochlorophyllide (Pchlide), i.e. Pchlide bound to protochlorophyllide oxidoreductase, which was shown in fluorescence spectra. The photoreduction was followed by the disruption of PLB structures, which started with the enlargement of PLB spheres and then their fragmentation into small balls as seen with AFM. Light-induced vesicle formation and the outgrowth of lamellar (pro)thylakoid membranes on the PLB surface were also confirmed by TEM analyses, and resulted in the apparent enlargement of the PLB diameter. The blue-shift of the fluorescence emission maximum of chlorophyllide observed for PLBs at room temperature after Pchlide photoreduction was completed within 25min. However, structural changes in PLBs were still observed after the completion of the blue-shift. The incubation of PLBs in darkness with HgCl2 also resulted in PLB enlargement and a loosening of their structure. AFM provides a unique opportunity to observe PLBs at a physiological temperature without the necessity of fixation.

Original languageEnglish
Pages (from-to)1217-1227
Number of pages11
JournalJournal of Plant Physiology
Volume170
Issue number14
DOIs
Publication statusPublished - Sep 15 2013

Fingerprint

Protochlorophyllide
protochlorophyllides
atomic force microscopy
Atomic Force Microscopy
Transmission Electron Microscopy
transmission electron microscopy
Triticum
fluorescence emission spectroscopy
Chlorophyllides
Fluorescence
fluorescence
etioplasts
chlorophyllides
Mercuric Chloride
wheat
Thylakoids
mica
Temperature
Darkness
Fluorescence Spectrometry

Keywords

  • AFM
  • Fluorescence spectra
  • PLB
  • Protochlorophyllide photoreduction
  • TEM

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Visualization and characterization of prolamellar bodies with atomic force microscopy. / Grzyb, Joanna M.; Solymosi, K.; Strzałka, Kazimierz; Mysliwa-Kurdziel, Beata.

In: Journal of Plant Physiology, Vol. 170, No. 14, 15.09.2013, p. 1217-1227.

Research output: Contribution to journalArticle

Grzyb, Joanna M. ; Solymosi, K. ; Strzałka, Kazimierz ; Mysliwa-Kurdziel, Beata. / Visualization and characterization of prolamellar bodies with atomic force microscopy. In: Journal of Plant Physiology. 2013 ; Vol. 170, No. 14. pp. 1217-1227.
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