Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy

N. Bokor, Keiichi Inoue, Satoshi Kogure, Masaaki Fujii, Makoto Sakai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A scanning visible-super-resolution microscope based on the saturation behaviour of transient fluorescence detected infrared (TFD-IR) spectroscopy is proposed. A Gaussian IR beam, a Gaussian visible beam and a Laguerre-Gaussian (LG) visible beam are used to obtain two separate two-color excitation fluorescence (2CF) images of the sample. The final image is obtained as the difference between the two recorded images. If the peak intensity of the LG beam is high enough to induce saturation in the fluorescence signal, the image can, in principle, have unlimited spatial resolution. A ∼3-fold improvement in transverse resolution over the visible diffraction limit (and far exceeding the IR diffraction limit) is easily achievable in present experimental setups.

Original languageEnglish
Pages (from-to)509-514
Number of pages6
JournalOptics Communications
Volume283
Issue number3
DOIs
Publication statusPublished - Feb 1 2010

Fingerprint

Fluorescence spectroscopy
Infrared spectroscopy
Microscopic examination
Diffraction
Fluorescence
infrared spectroscopy
microscopy
Infrared radiation
fluorescence
Gaussian beams
Microscopes
Color
Scanning
saturation
diffraction
spatial resolution
microscopes
color
scanning
excitation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Physical and Theoretical Chemistry

Cite this

Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy. / Bokor, N.; Inoue, Keiichi; Kogure, Satoshi; Fujii, Masaaki; Sakai, Makoto.

In: Optics Communications, Vol. 283, No. 3, 01.02.2010, p. 509-514.

Research output: Contribution to journalArticle

Bokor, N. ; Inoue, Keiichi ; Kogure, Satoshi ; Fujii, Masaaki ; Sakai, Makoto. / Visible-super-resolution infrared microscopy using saturated transient fluorescence detected infrared spectroscopy. In: Optics Communications. 2010 ; Vol. 283, No. 3. pp. 509-514.
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