Virulence traits of inpatient campylobacter jejuni isolates, and a transcriptomic approach to identify potential genes maintaining intracellular survival

Judit K. Kovács, Alysia Cox, Bettina Schweitzer, Gergely Maróti, Tamás Kovács, Hajnalka Fenyvesi, Levente Emody, György Schneider

Research output: Contribution to journalArticle

Abstract

There are still major gaps in our understanding of the bacterial factors that influence the outcomes of human Campylobacter jejuni infection. The aim of this study was to compare the virulence-associated features of 192 human C. jejuni strains isolated from hospitalized patients with diarrhoea (150/192, 78.1%), bloody diarrhoea (23/192, 11.9%), gastroenteritis (3/192, 1.6%), ulcerative colitis (3/192, 1.5%), and stomach ache (2/192, 1.0%). Traits were analysed with genotypic and phenotypic methods, including PCR and extracellular matrix protein (ECMP) binding, adhesion, and invasion capacities. Results were studied alongside patient symptoms, but no distinct links with them could be determined. Since the capacity of C. jejuni to invade host epithelial cells is one of its most enigmatic attributes, a high throughput transcriptomic analysis was performed in the third hour of internalization with a C. jejuni strain originally isolated from bloody diarrhoea. Characteristic groups of genes were significantly upregulated, outlining a survival strategy of internalized C. jejuni comprising genes related (1) to oxidative stress; (2) to a protective sheath formed by the capsule, LOS, N-, and O- glycosylation systems; (3) to dynamic metabolic activity supported by different translocases and the membrane-integrated component of the flagellar apparatus; and (4) to hitherto unknown genes.

Original languageEnglish
Article number531
JournalMicroorganisms
Volume8
Issue number4
DOIs
Publication statusPublished - 2020

Keywords

  • Campylobacter jejuni
  • Clinical isolates
  • Genomic approach
  • Intracellular survival
  • Transcriptomic analysis
  • Virulence potential

ASJC Scopus subject areas

  • Microbiology
  • Virology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Virulence traits of inpatient campylobacter jejuni isolates, and a transcriptomic approach to identify potential genes maintaining intracellular survival'. Together they form a unique fingerprint.

  • Cite this