Virulence of two entomophthoralean fungi, pandora neoaphidis and entomophthora planchoniana, to their conspecific (Sitobion avenae) and heterospecific (rhopalosiphum padi) aphid hosts

Ibtissem Ben Fekih, Annette Bruun Jensen, Sonia Boukhris-Bouhachem, G. Pozsgai, Salah Rezgui, Christopher Rensing, Jørgen Eilenberg

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pandora neoaphidis and Entomophthora planchoniana (phylum Entomophthoromycota) are important fungal pathogens on cereal aphids, Sitobion avenae and Rhopalosiphum padi. Here, we evaluated and compared for the first time the virulence of these two fungi, both produced in S. avenae cadavers, against the two aphid species subjected to the same exposure. Two laboratory bioassays were carried out using a method imitating entomophthoralean transmission in the field. Healthy colonies of the two aphid species were exposed to the same conidial shower of P. neoaphidis or E. planchoniana, in both cases from a cadaver of S. avenae. The experiments were performed under LD 18:6 h at 21 °C and a successful transmission was monitored for a period of nine days after initial exposure. Susceptibility of both S. avenae and R. padi to fungal infection showed a sigmoid trend. The fitted nonlinear model showed that the conspecific host, S. avenae, was more susceptible to E. planchoniana infection than the heterospecific host R. padi, was. In the case of P. neoaphidis, LT50 for S. avenae was 5.0 days compared to 5.9 days for R. padi. For E. planchoniana, the LT50 for S. avenae was 4.9 days, while the measured infection level in R. padi was always below 50 percent. Our results suggest that transmission from conspecific aphid host to heterospecific aphid host can occur in the field, but with expected highest transmission success to the conspecific host.

Original languageEnglish
Article number54
Pages (from-to)1-11
Number of pages11
JournalInsects
Volume10
Issue number2
DOIs
Publication statusPublished - Feb 1 2019

Fingerprint

Erynia neoaphidis
Entomophthora
Sitobion avenae
Rhopalosiphum padi
Aphidoidea
virulence
fungi
infection
nonlinear models
bioassays
pathogens

Keywords

  • Aphids
  • Entomophthora planchoniana
  • Pandora neoaphidis
  • Virulence

ASJC Scopus subject areas

  • Insect Science

Cite this

Virulence of two entomophthoralean fungi, pandora neoaphidis and entomophthora planchoniana, to their conspecific (Sitobion avenae) and heterospecific (rhopalosiphum padi) aphid hosts. / Fekih, Ibtissem Ben; Jensen, Annette Bruun; Boukhris-Bouhachem, Sonia; Pozsgai, G.; Rezgui, Salah; Rensing, Christopher; Eilenberg, Jørgen.

In: Insects, Vol. 10, No. 2, 54, 01.02.2019, p. 1-11.

Research output: Contribution to journalArticle

Fekih, Ibtissem Ben ; Jensen, Annette Bruun ; Boukhris-Bouhachem, Sonia ; Pozsgai, G. ; Rezgui, Salah ; Rensing, Christopher ; Eilenberg, Jørgen. / Virulence of two entomophthoralean fungi, pandora neoaphidis and entomophthora planchoniana, to their conspecific (Sitobion avenae) and heterospecific (rhopalosiphum padi) aphid hosts. In: Insects. 2019 ; Vol. 10, No. 2. pp. 1-11.
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abstract = "Pandora neoaphidis and Entomophthora planchoniana (phylum Entomophthoromycota) are important fungal pathogens on cereal aphids, Sitobion avenae and Rhopalosiphum padi. Here, we evaluated and compared for the first time the virulence of these two fungi, both produced in S. avenae cadavers, against the two aphid species subjected to the same exposure. Two laboratory bioassays were carried out using a method imitating entomophthoralean transmission in the field. Healthy colonies of the two aphid species were exposed to the same conidial shower of P. neoaphidis or E. planchoniana, in both cases from a cadaver of S. avenae. The experiments were performed under LD 18:6 h at 21 °C and a successful transmission was monitored for a period of nine days after initial exposure. Susceptibility of both S. avenae and R. padi to fungal infection showed a sigmoid trend. The fitted nonlinear model showed that the conspecific host, S. avenae, was more susceptible to E. planchoniana infection than the heterospecific host R. padi, was. In the case of P. neoaphidis, LT50 for S. avenae was 5.0 days compared to 5.9 days for R. padi. For E. planchoniana, the LT50 for S. avenae was 4.9 days, while the measured infection level in R. padi was always below 50 percent. Our results suggest that transmission from conspecific aphid host to heterospecific aphid host can occur in the field, but with expected highest transmission success to the conspecific host.",
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AU - Fekih, Ibtissem Ben

AU - Jensen, Annette Bruun

AU - Boukhris-Bouhachem, Sonia

AU - Pozsgai, G.

AU - Rezgui, Salah

AU - Rensing, Christopher

AU - Eilenberg, Jørgen

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N2 - Pandora neoaphidis and Entomophthora planchoniana (phylum Entomophthoromycota) are important fungal pathogens on cereal aphids, Sitobion avenae and Rhopalosiphum padi. Here, we evaluated and compared for the first time the virulence of these two fungi, both produced in S. avenae cadavers, against the two aphid species subjected to the same exposure. Two laboratory bioassays were carried out using a method imitating entomophthoralean transmission in the field. Healthy colonies of the two aphid species were exposed to the same conidial shower of P. neoaphidis or E. planchoniana, in both cases from a cadaver of S. avenae. The experiments were performed under LD 18:6 h at 21 °C and a successful transmission was monitored for a period of nine days after initial exposure. Susceptibility of both S. avenae and R. padi to fungal infection showed a sigmoid trend. The fitted nonlinear model showed that the conspecific host, S. avenae, was more susceptible to E. planchoniana infection than the heterospecific host R. padi, was. In the case of P. neoaphidis, LT50 for S. avenae was 5.0 days compared to 5.9 days for R. padi. For E. planchoniana, the LT50 for S. avenae was 4.9 days, while the measured infection level in R. padi was always below 50 percent. Our results suggest that transmission from conspecific aphid host to heterospecific aphid host can occur in the field, but with expected highest transmission success to the conspecific host.

AB - Pandora neoaphidis and Entomophthora planchoniana (phylum Entomophthoromycota) are important fungal pathogens on cereal aphids, Sitobion avenae and Rhopalosiphum padi. Here, we evaluated and compared for the first time the virulence of these two fungi, both produced in S. avenae cadavers, against the two aphid species subjected to the same exposure. Two laboratory bioassays were carried out using a method imitating entomophthoralean transmission in the field. Healthy colonies of the two aphid species were exposed to the same conidial shower of P. neoaphidis or E. planchoniana, in both cases from a cadaver of S. avenae. The experiments were performed under LD 18:6 h at 21 °C and a successful transmission was monitored for a period of nine days after initial exposure. Susceptibility of both S. avenae and R. padi to fungal infection showed a sigmoid trend. The fitted nonlinear model showed that the conspecific host, S. avenae, was more susceptible to E. planchoniana infection than the heterospecific host R. padi, was. In the case of P. neoaphidis, LT50 for S. avenae was 5.0 days compared to 5.9 days for R. padi. For E. planchoniana, the LT50 for S. avenae was 4.9 days, while the measured infection level in R. padi was always below 50 percent. Our results suggest that transmission from conspecific aphid host to heterospecific aphid host can occur in the field, but with expected highest transmission success to the conspecific host.

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