Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species

B. Ujvári, L. Makrai, T. Magyar

Research output: Contribution to journalArticle

Abstract

This study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA)sequence of Pasteurella multocida isolates (n = 180)from different locations in Hungary, from various host species, including humans. When combining capsular types with LPS genotypes, eight capsule - LPS genotype combinations were detected. A: L3 was the most dominant in bovine and porcine isolates, A: L1 in feline and human isolates, while D: L3 was the most common among strains from small ruminants. The P. multocida toxin encoding gene toxA was highly prevalent among small ruminant and porcine strains, while in human, feline and bovine isolates it could not be detected. Combination of the tested virulence associated genes (hgbA, nanH, hgbB, tbpA, pfhA, hsf1, hsf2, tadD, ptfA)classified our P. multocida isolates into 13 different virulence gene profiles (VGPs). These VGPs showed an association with host species. Analysis of the ompA sequence data confirmed this distribution by host species, which may indicate that host adaptation is taking place. The typing scheme used in this study may be useful in epidemiological investigations.

Original languageEnglish
Pages (from-to)190-195
Number of pages6
JournalVeterinary Microbiology
Volume233
DOIs
Publication statusPublished - Jun 1 2019

Fingerprint

Pasteurella multocida
Sequence Analysis
Virulence
virulence
sequence analysis
Genes
genes
Felidae
Genotype
Ruminants
small ruminants
Capsules
genotype
Swine
cats
swine
Hungary
outer membrane proteins
cattle
Membrane Proteins

Keywords

  • Molecular typing
  • ompA
  • Pasteurella multocida
  • Virulence-Associated genes

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species. / Ujvári, B.; Makrai, L.; Magyar, T.

In: Veterinary Microbiology, Vol. 233, 01.06.2019, p. 190-195.

Research output: Contribution to journalArticle

@article{3caea7daf0d144de954fe2d336f9e0ae,
title = "Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species",
abstract = "This study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA)sequence of Pasteurella multocida isolates (n = 180)from different locations in Hungary, from various host species, including humans. When combining capsular types with LPS genotypes, eight capsule - LPS genotype combinations were detected. A: L3 was the most dominant in bovine and porcine isolates, A: L1 in feline and human isolates, while D: L3 was the most common among strains from small ruminants. The P. multocida toxin encoding gene toxA was highly prevalent among small ruminant and porcine strains, while in human, feline and bovine isolates it could not be detected. Combination of the tested virulence associated genes (hgbA, nanH, hgbB, tbpA, pfhA, hsf1, hsf2, tadD, ptfA)classified our P. multocida isolates into 13 different virulence gene profiles (VGPs). These VGPs showed an association with host species. Analysis of the ompA sequence data confirmed this distribution by host species, which may indicate that host adaptation is taking place. The typing scheme used in this study may be useful in epidemiological investigations.",
keywords = "Molecular typing, ompA, Pasteurella multocida, Virulence-Associated genes",
author = "B. Ujv{\'a}ri and L. Makrai and T. Magyar",
year = "2019",
month = "6",
day = "1",
doi = "10.1016/j.vetmic.2019.05.005",
language = "English",
volume = "233",
pages = "190--195",
journal = "Veterinary Microbiology",
issn = "0378-1135",
publisher = "Elsevier",

}

TY - JOUR

T1 - Virulence gene profiling and ompA sequence analysis of Pasteurella multocida and their correlation with host species

AU - Ujvári, B.

AU - Makrai, L.

AU - Magyar, T.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - This study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA)sequence of Pasteurella multocida isolates (n = 180)from different locations in Hungary, from various host species, including humans. When combining capsular types with LPS genotypes, eight capsule - LPS genotype combinations were detected. A: L3 was the most dominant in bovine and porcine isolates, A: L1 in feline and human isolates, while D: L3 was the most common among strains from small ruminants. The P. multocida toxin encoding gene toxA was highly prevalent among small ruminant and porcine strains, while in human, feline and bovine isolates it could not be detected. Combination of the tested virulence associated genes (hgbA, nanH, hgbB, tbpA, pfhA, hsf1, hsf2, tadD, ptfA)classified our P. multocida isolates into 13 different virulence gene profiles (VGPs). These VGPs showed an association with host species. Analysis of the ompA sequence data confirmed this distribution by host species, which may indicate that host adaptation is taking place. The typing scheme used in this study may be useful in epidemiological investigations.

AB - This study describes the prevalence of capsule biosynthesis genes, LPS genotypes, virulence associated genes and the analysis of the outer membrane protein (ompA)sequence of Pasteurella multocida isolates (n = 180)from different locations in Hungary, from various host species, including humans. When combining capsular types with LPS genotypes, eight capsule - LPS genotype combinations were detected. A: L3 was the most dominant in bovine and porcine isolates, A: L1 in feline and human isolates, while D: L3 was the most common among strains from small ruminants. The P. multocida toxin encoding gene toxA was highly prevalent among small ruminant and porcine strains, while in human, feline and bovine isolates it could not be detected. Combination of the tested virulence associated genes (hgbA, nanH, hgbB, tbpA, pfhA, hsf1, hsf2, tadD, ptfA)classified our P. multocida isolates into 13 different virulence gene profiles (VGPs). These VGPs showed an association with host species. Analysis of the ompA sequence data confirmed this distribution by host species, which may indicate that host adaptation is taking place. The typing scheme used in this study may be useful in epidemiological investigations.

KW - Molecular typing

KW - ompA

KW - Pasteurella multocida

KW - Virulence-Associated genes

UR - http://www.scopus.com/inward/record.url?scp=85065540800&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065540800&partnerID=8YFLogxK

U2 - 10.1016/j.vetmic.2019.05.005

DO - 10.1016/j.vetmic.2019.05.005

M3 - Article

VL - 233

SP - 190

EP - 195

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -