Virological failure of intralesional cidofovir therapy in recurrent respiratory papillomatosis is not associated with genetic or epigenetic changes of HPV11: Complete genome comparison of sequential isolates

Tamás Gáll, Andrea Kis, Eniko Fehér, Lajos Gergely, Krisztina Szarka

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Five sequential human papillomavirus type 11 (HPV11) positive samples collected from an aggressive juvenile onset recurrent respiratory papillomatosis before, during and after intralesional cidofovir therapy leading to virological failure after initial response were analyzed. Sequencing of the complete genome as well as methylation analysis by bisulfate modification and sequencing of the long control region (LCR) were performed to seek for genetic and epigenetic changes as a possible background for therapy failure. Single-strand conformation polymorphism of E1, E2, E6, E7 and LCR was used to exclude the presence of multiple HPV11 infection. All five complete genomes were identical and all four E2 binding sites in the LCR were uniformly unmethylated in all five genomes. Thus the virological failure was not due to virological factors suggesting that cidofovir action may depend more heavily on the host.

Original languageEnglish
Pages (from-to)356-358
Number of pages3
JournalAntiviral Research
Volume92
Issue number2
DOIs
Publication statusPublished - Nov 1 2011

Keywords

  • Antiviral resistance
  • Complete genome
  • LCR methylation
  • Laryngeal papillomatosis
  • Therapeutic response

ASJC Scopus subject areas

  • Pharmacology
  • Virology

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