Vigorous growth of fusion products allows highly efficient selection of interspecific potato somatic hybrids

molecular proofs

Zs Polgár, J. Preiszner, D. Dudits, A. Fehér

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

An early identification of fusion products was based on the presumed vigorous growth of hybrid calluses after fusion between Solanum brevidens and S. tuberosum leaf protoplasts. The S. brevidens protoplasts were unable to form multicellular colonies under the applied culture conditions. Three size groups of calluses were separated and analyzed at two different early phases of culture period. "Squash blot" hybridization with a S. brevidens specific repetitive DNA probe showed that the group of the largest calluses consisted of putative somatic hybrids with a frequency of 80-100% in three independent experiments. Furthermore, approximately 80-95% of the middle sized calluses and 33-90% of the smallest ones were shown to be hybrid. The unexpectedly high percentage of fusion products, even in the case of the smallest calluses, may result from the suppression of the development of parental potato colonies in cultures with mixed cell population. Till this time 120 independent colonies selected as putative hybrids have been regenerated into plants. All of them exhibited hybrid phenotype, and their hybrid origin was proved by cytological and restriction fragment length polymorphism analyses.

Original languageEnglish
Pages (from-to)399-402
Number of pages4
JournalPlant Cell Reports
Volume12
Issue number7-8
DOIs
Publication statusPublished - May 1993

Fingerprint

Solanum palustre
potatoes
callus
protoplasts
squashes
DNA probes
group size
Solanum tuberosum
restriction fragment length polymorphism
hybridization
phenotype
leaves
cells

Keywords

  • hybrid vigor
  • potato
  • Solanum
  • somatic hybrids
  • species-specific repetitive DNA

ASJC Scopus subject areas

  • Plant Science

Cite this

Vigorous growth of fusion products allows highly efficient selection of interspecific potato somatic hybrids : molecular proofs. / Polgár, Zs; Preiszner, J.; Dudits, D.; Fehér, A.

In: Plant Cell Reports, Vol. 12, No. 7-8, 05.1993, p. 399-402.

Research output: Contribution to journalArticle

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