Video-fluorimetric evaluation of human blood serum electrophoretograms

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Abstract

The aim of the present study was to develop a very fast and reliable staining method for the evaluation of blood serum electrophoretograms. The fluorescent dye applied, fluorescamine, was found superior to most of the conventional protein dyes. The staining of the developed electrophoretograms is very fast and no fixation procedure is required. The linearity of the staining and detection method within the tested protein concentration range has been proven. Video-fluorimetric evaluation of the fluorescamine-stained electrophoretograms was established. The video-fluorimeter provides data in a very convenient, digital form and is capable of evaluating large numbers of chromatograms with high speed. Moreover, the evaluation of the results can be automatized by transferring the data from the instrument into an online connected minicomputer.

Original languageEnglish
Pages (from-to)199-203
Number of pages5
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume15
Issue number3
Publication statusPublished - 1980

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Fluorescamine
Staining and Labeling
Serum
Minicomputers
Fluorescent Dyes
Proteins
Coloring Agents

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "The aim of the present study was to develop a very fast and reliable staining method for the evaluation of blood serum electrophoretograms. The fluorescent dye applied, fluorescamine, was found superior to most of the conventional protein dyes. The staining of the developed electrophoretograms is very fast and no fixation procedure is required. The linearity of the staining and detection method within the tested protein concentration range has been proven. Video-fluorimetric evaluation of the fluorescamine-stained electrophoretograms was established. The video-fluorimeter provides data in a very convenient, digital form and is capable of evaluating large numbers of chromatograms with high speed. Moreover, the evaluation of the results can be automatized by transferring the data from the instrument into an online connected minicomputer.",
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AB - The aim of the present study was to develop a very fast and reliable staining method for the evaluation of blood serum electrophoretograms. The fluorescent dye applied, fluorescamine, was found superior to most of the conventional protein dyes. The staining of the developed electrophoretograms is very fast and no fixation procedure is required. The linearity of the staining and detection method within the tested protein concentration range has been proven. Video-fluorimetric evaluation of the fluorescamine-stained electrophoretograms was established. The video-fluorimeter provides data in a very convenient, digital form and is capable of evaluating large numbers of chromatograms with high speed. Moreover, the evaluation of the results can be automatized by transferring the data from the instrument into an online connected minicomputer.

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