Very low sperm-egg ratios result in successful fertilization using cryopreserved sperm in the Adriatic grayling (Thymallus thymallus)

Ákos Horváth, Zoltán Bokor, Gergely Bernáth, Zsolt Csenki, Ada Gorjan, Maria Paz Herráez, Béla Urbányi, Dušan Jesenšek

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The effect of four sperm-egg ratios on the fertilizing capacity of cryopreserved sperm of the genetically distinct Adriatic grayling (Thymallus thymallus) was investigated. Sperm was diluted at a ratio of 1:1 in an extender composed of 200mM glucose, 40mM KCl, 30mM Tris (pH8.0) and 10% methanol and frozen in the vapor of liquid nitrogen. Fertilization tests were conducted with sperm-egg ratios ranging from 103 to 5×104 spermatozoa to one egg. Percentages of eyed eggs (60±7%) and hatched larvae (53±7%) were not significantly different from the control (68±3% eyed eggs and 55±3% hatched larvae) with the sperm-egg ratio of 5×104 spermatozoa to one egg. Cryopreserved sperm retained its fertilizing capacity even with the lowest tested sperm-egg ratio.

Original languageEnglish
Pages (from-to)75-77
Number of pages3
Publication statusPublished - Jan 1 2015



  • Cryopreservation
  • Fertilization
  • Grayling
  • Sperm
  • Sperm-egg ratios

ASJC Scopus subject areas

  • Aquatic Science

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