Very early effect of DMBA and MNU on MicroRNA expression

Krisztina Juhász, Katalin Gombos, Mónika Szirmai, Katalin Gocze, Veronika Wolher, Péter Révész, Ingrid Magda, Andor Sebestyén, Árpád Németh, I. Ember

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: microRNA expression profile analysis provides evaluation of the early stages of carcinogenesis. This study focuses on early alteration of miRNA expression after treatment with different carcinogens. Materials and Methods: Mice were intraperitoneally injected with one dose of 7,12-dimethylbenz(α) anthracene (DMBA) and N-methyl-N-nitrosourea (MNU). The expression of miRNAs were analyzed 3 and 6 hours after the treatment, using quantitative real-time-polymerase chain reaction. Results: Underexpression of miR-34a and miR-155 were detected in the liver, spleen and kidneys at 3 and 6 hours after MNU treatment. In the lungs and kidneys, the expression of miR-21 was significantly elevated 6 hours after DMBA treatment, while in the liver, MNU induced higher expression levels of miR-21 at 3 and 6 hours compared to treatment with DMBA. Conclusion: The different response of miRNAs to carcinogens emphasizes their possible role as potential epidemiological biomarkers in early phases of environmental tumorigenesis.

Original languageEnglish
Pages (from-to)113-118
Number of pages6
JournalIn Vivo
Volume27
Issue number1
Publication statusPublished - Jan 2013

Fingerprint

MicroRNAs
Carcinogens
Liver
Carcinogenesis
Kidney
Methylnitrosourea
Polymerase chain reaction
Biomarkers
Real-Time Polymerase Chain Reaction
Spleen
Lung
anthracene

Keywords

  • 7,12-Dimethylbenz(α)anthracene
  • Chemical carcinogenesis
  • MicroRNA
  • N-methyl-N-nitrosourea

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Juhász, K., Gombos, K., Szirmai, M., Gocze, K., Wolher, V., Révész, P., ... Ember, I. (2013). Very early effect of DMBA and MNU on MicroRNA expression. In Vivo, 27(1), 113-118.

Very early effect of DMBA and MNU on MicroRNA expression. / Juhász, Krisztina; Gombos, Katalin; Szirmai, Mónika; Gocze, Katalin; Wolher, Veronika; Révész, Péter; Magda, Ingrid; Sebestyén, Andor; Németh, Árpád; Ember, I.

In: In Vivo, Vol. 27, No. 1, 01.2013, p. 113-118.

Research output: Contribution to journalArticle

Juhász, K, Gombos, K, Szirmai, M, Gocze, K, Wolher, V, Révész, P, Magda, I, Sebestyén, A, Németh, Á & Ember, I 2013, 'Very early effect of DMBA and MNU on MicroRNA expression', In Vivo, vol. 27, no. 1, pp. 113-118.
Juhász K, Gombos K, Szirmai M, Gocze K, Wolher V, Révész P et al. Very early effect of DMBA and MNU on MicroRNA expression. In Vivo. 2013 Jan;27(1):113-118.
Juhász, Krisztina ; Gombos, Katalin ; Szirmai, Mónika ; Gocze, Katalin ; Wolher, Veronika ; Révész, Péter ; Magda, Ingrid ; Sebestyén, Andor ; Németh, Árpád ; Ember, I. / Very early effect of DMBA and MNU on MicroRNA expression. In: In Vivo. 2013 ; Vol. 27, No. 1. pp. 113-118.
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