Versatile insertion plasmids for targeted genome manipulations in bacteria: Isolation, deletion, and rescue of the pathogenicity island LEE of the Escherichia coli O157:H7 genome

György Pósfai, Michael D. Koob, Heather A. Kirkpatrick, Frederick R. Blattner

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

A system of versatile insertion plasmids was constructed that permits efficient delivery of the target sites of an ultra-rare-cutting endonuclease and the recombinase FLP into preselected sites of the bacterial genome. With the help of this system, the pathogenicity island LEE of the Escherichia coli O157:H7 genome was excised and isolated in vitro, deleted in vivo, rescued as a plasmid, and transferred into another strain.

Original languageEnglish
Pages (from-to)4426-4428
Number of pages3
JournalJournal of bacteriology
Volume179
Issue number13
DOIs
Publication statusPublished - Jul 1997

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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