Venodilatory effect of vascular endothelial growth factor on rat gingiva

Milan Gyurkovics, Zs. Lohinai, Adrienne Gyórfi, Iván Iványi, Ibolya Süveges, Mária Kónya, Csaba Bodor, A. Székely, E. Dinya, Arpád Fazekas, L. Rosivall

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Abstract

Background: Endothelial cell proliferation, angiogenesis, and increased vascular permeability are among the effects of vascular endothelial growth factor (VEGF) in various organs. However, the effects of VEGF on gingival hemodynamics, especially on venules, have not been thoroughly investigated. This study investigated the acute circulatory effects of VEGF on rat gingival venules. Methods: Fifty-six anesthetized rats were divided into five study groups; each rat received 10 μl of experimental solution dripped onto the lower interincisal gingiva. The groups included: 1) saline control (after the experiment, gingiva was excised for VEGF receptor 2 [VEGFR2] immunohistochemis- try); 2) VEGF (0.1, 1, 10, or 50 μ/ml); 3) VEGF2 receptor antagonist 5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro- 2-methyl-phenol-hydrochloride (ZM323881; 20 μ/ml); 4) ZM323881 (20 mg/ml) followed by VEGF application (50 μ/ml after 15 minutes); and 5) VEGF (10 μ/ml), these rats were premedicated with nitric oxide (NO) synthase blocker (NG-nitro-L-arginine- methyl-ester [L-NAME]; 1 mg/ ml in drinking water) for 1 week before the experiment. Changes in gingival superficial venule diameter were measured by vital microscopy prior to and 1, 5, 15, 30, and 60 minutes after the administration ofthe experimental solutions. Results: VEGF dose-dependently increased the venular diameter compared to saline. ZM323881 alone did not cause any alteration. Premedication with ZM323881 or L-NAME decreased the dilatory effects of VEGF. VEGFR2 immunohisto- chemical labeling was observed in the wall of the venules. Conclusions: There is no remarkable VEGF production under physiologic circumstances in rat gingiva, but VEGF is able to increase gingival blood flow through the activation of VEGF2 receptors. Furthermore, NO release may contribute to VEGF's vasodilatory effect.

Original languageEnglish
Pages (from-to)1518-1523
Number of pages6
JournalJournal of Periodontology
Volume80
Issue number9
DOIs
Publication statusPublished - Sep 2009

Fingerprint

Gingiva
Vascular Endothelial Growth Factor A
Venules
NG-Nitroarginine Methyl Ester
Vascular Endothelial Growth Factor Receptor-2
Vascular Endothelial Growth Factor Receptor
Premedication
Capillary Permeability
Phenol
Nitric Oxide Synthase
Drinking Water
Microscopy
Nitric Oxide
Endothelial Cells
Hemodynamics
Immunohistochemistry
Cell Proliferation
ZM323881

Keywords

  • Biology
  • Cytokines
  • Growth factors
  • Nitric oxide
  • Vascular

ASJC Scopus subject areas

  • Periodontics

Cite this

Venodilatory effect of vascular endothelial growth factor on rat gingiva. / Gyurkovics, Milan; Lohinai, Zs.; Gyórfi, Adrienne; Iványi, Iván; Süveges, Ibolya; Kónya, Mária; Bodor, Csaba; Székely, A.; Dinya, E.; Fazekas, Arpád; Rosivall, L.

In: Journal of Periodontology, Vol. 80, No. 9, 09.2009, p. 1518-1523.

Research output: Contribution to journalArticle

Gyurkovics, M, Lohinai, Z, Gyórfi, A, Iványi, I, Süveges, I, Kónya, M, Bodor, C, Székely, A, Dinya, E, Fazekas, A & Rosivall, L 2009, 'Venodilatory effect of vascular endothelial growth factor on rat gingiva', Journal of Periodontology, vol. 80, no. 9, pp. 1518-1523. https://doi.org/10.1902/jop.2009.080651
Gyurkovics, Milan ; Lohinai, Zs. ; Gyórfi, Adrienne ; Iványi, Iván ; Süveges, Ibolya ; Kónya, Mária ; Bodor, Csaba ; Székely, A. ; Dinya, E. ; Fazekas, Arpád ; Rosivall, L. / Venodilatory effect of vascular endothelial growth factor on rat gingiva. In: Journal of Periodontology. 2009 ; Vol. 80, No. 9. pp. 1518-1523.
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abstract = "Background: Endothelial cell proliferation, angiogenesis, and increased vascular permeability are among the effects of vascular endothelial growth factor (VEGF) in various organs. However, the effects of VEGF on gingival hemodynamics, especially on venules, have not been thoroughly investigated. This study investigated the acute circulatory effects of VEGF on rat gingival venules. Methods: Fifty-six anesthetized rats were divided into five study groups; each rat received 10 μl of experimental solution dripped onto the lower interincisal gingiva. The groups included: 1) saline control (after the experiment, gingiva was excised for VEGF receptor 2 [VEGFR2] immunohistochemis- try); 2) VEGF (0.1, 1, 10, or 50 μ/ml); 3) VEGF2 receptor antagonist 5-((7-benzyloxyquinazolin-4-yl)amino)-4-fluoro- 2-methyl-phenol-hydrochloride (ZM323881; 20 μ/ml); 4) ZM323881 (20 mg/ml) followed by VEGF application (50 μ/ml after 15 minutes); and 5) VEGF (10 μ/ml), these rats were premedicated with nitric oxide (NO) synthase blocker (NG-nitro-L-arginine- methyl-ester [L-NAME]; 1 mg/ ml in drinking water) for 1 week before the experiment. Changes in gingival superficial venule diameter were measured by vital microscopy prior to and 1, 5, 15, 30, and 60 minutes after the administration ofthe experimental solutions. Results: VEGF dose-dependently increased the venular diameter compared to saline. ZM323881 alone did not cause any alteration. Premedication with ZM323881 or L-NAME decreased the dilatory effects of VEGF. VEGFR2 immunohisto- chemical labeling was observed in the wall of the venules. Conclusions: There is no remarkable VEGF production under physiologic circumstances in rat gingiva, but VEGF is able to increase gingival blood flow through the activation of VEGF2 receptors. Furthermore, NO release may contribute to VEGF's vasodilatory effect.",
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AU - Süveges, Ibolya

AU - Kónya, Mária

AU - Bodor, Csaba

AU - Székely, A.

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