Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition

Katharina J. Jetzschmann, Gyula Jágerszki, Decha Dechtrirat, Aysu Yarman, Nenad Gajovic-Eichelmann, Hans Detlev Gilsing, Burkhard Schulz, Róbert E. Gyurcsányi, Frieder W. Scheller

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25 Citations (Scopus)


Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed of a propidium-terminated self-assembled monolayer (Prop-SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer of a novel carboxylate-modified derivative of 3,4-propylenedioxythiophene. The rebinding of the AChE to the MIP/Prop-SAM nanofilm covered electrode is detected by measuring in situ the enzymatic activity. The oxidative current of the released thiocholine is dependent on the AChE concentration from ≈0.04 × 10-6 to 0.4 × 10-6m. An imprinting factor of 9.9 is obtained for the hybrid MIP, which is among the best values reported for protein imprinting. The dissociation constant characterizing the strength of the MIP-AChE binding is 4.2 × 10-7m indicating the dominant role of the PAS-Prop-SAM interaction, while the benefit of the MIP nanofilm covering the Prop-SAM layer is the effective suppression of the cross-reactivity toward competing proteins as compared with the Prop-SAM. The threefold selectivity gain provided by i) the "shape-specific" MIP filter, ii) the propidium-SAM, iii) signal generation only by the AChE bound to the nanofilm shows promise for assessing AChE activity levels in cerebrospinal fluid.

Original languageEnglish
Pages (from-to)5178-5183
Number of pages6
JournalAdvanced Functional Materials
Issue number32
Publication statusPublished - Aug 1 2015


  • acetylcholinesterase
  • biomimetic sensors
  • molecularly imprinted electropolymers
  • peripheral anionic site
  • propidium

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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  • Cite this

    Jetzschmann, K. J., Jágerszki, G., Dechtrirat, D., Yarman, A., Gajovic-Eichelmann, N., Gilsing, H. D., Schulz, B., Gyurcsányi, R. E., & Scheller, F. W. (2015). Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. Advanced Functional Materials, 25(32), 5178-5183.