Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition

Katharina J. Jetzschmann, Gyula Jágerszki, Decha Dechtrirat, Aysu Yarman, Nenad Gajovic-Eichelmann, Hans Detlev Gilsing, Burkhard Schulz, R. Gyurcsányi, Frieder W. Scheller

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed of a propidium-terminated self-assembled monolayer (Prop-SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer of a novel carboxylate-modified derivative of 3,4-propylenedioxythiophene. The rebinding of the AChE to the MIP/Prop-SAM nanofilm covered electrode is detected by measuring in situ the enzymatic activity. The oxidative current of the released thiocholine is dependent on the AChE concentration from ≈0.04 × 10-6 to 0.4 × 10-6m. An imprinting factor of 9.9 is obtained for the hybrid MIP, which is among the best values reported for protein imprinting. The dissociation constant characterizing the strength of the MIP-AChE binding is 4.2 × 10-7m indicating the dominant role of the PAS-Prop-SAM interaction, while the benefit of the MIP nanofilm covering the Prop-SAM layer is the effective suppression of the cross-reactivity toward competing proteins as compared with the Prop-SAM. The threefold selectivity gain provided by i) the "shape-specific" MIP filter, ii) the propidium-SAM, iii) signal generation only by the AChE bound to the nanofilm shows promise for assessing AChE activity levels in cerebrospinal fluid.

Original languageEnglish
Pages (from-to)5178-5183
Number of pages6
JournalAdvanced Functional Materials
Volume25
Issue number32
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

Acetylcholinesterase
Propidium
Self assembled monolayers
Polymers
polymers
cerebrospinal fluid
Thiocholine
Cerebrospinal fluid
proteins
Proteins
carboxylates
coverings
reactivity
selectivity
retarding
dissociation
Derivatives
filters
Electrodes
electrodes

Keywords

  • acetylcholinesterase
  • biomimetic sensors
  • molecularly imprinted electropolymers
  • peripheral anionic site
  • propidium

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

Cite this

Jetzschmann, K. J., Jágerszki, G., Dechtrirat, D., Yarman, A., Gajovic-Eichelmann, N., Gilsing, H. D., ... Scheller, F. W. (2015). Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. Advanced Functional Materials, 25(32), 5178-5183. https://doi.org/10.1002/adfm.201501900

Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. / Jetzschmann, Katharina J.; Jágerszki, Gyula; Dechtrirat, Decha; Yarman, Aysu; Gajovic-Eichelmann, Nenad; Gilsing, Hans Detlev; Schulz, Burkhard; Gyurcsányi, R.; Scheller, Frieder W.

In: Advanced Functional Materials, Vol. 25, No. 32, 01.08.2015, p. 5178-5183.

Research output: Contribution to journalArticle

Jetzschmann, KJ, Jágerszki, G, Dechtrirat, D, Yarman, A, Gajovic-Eichelmann, N, Gilsing, HD, Schulz, B, Gyurcsányi, R & Scheller, FW 2015, 'Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition', Advanced Functional Materials, vol. 25, no. 32, pp. 5178-5183. https://doi.org/10.1002/adfm.201501900
Jetzschmann KJ, Jágerszki G, Dechtrirat D, Yarman A, Gajovic-Eichelmann N, Gilsing HD et al. Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. Advanced Functional Materials. 2015 Aug 1;25(32):5178-5183. https://doi.org/10.1002/adfm.201501900
Jetzschmann, Katharina J. ; Jágerszki, Gyula ; Dechtrirat, Decha ; Yarman, Aysu ; Gajovic-Eichelmann, Nenad ; Gilsing, Hans Detlev ; Schulz, Burkhard ; Gyurcsányi, R. ; Scheller, Frieder W. / Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. In: Advanced Functional Materials. 2015 ; Vol. 25, No. 32. pp. 5178-5183.
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