Variations in respiratory distress characterize the acute agonal period during heroin overdose death: Relevance to postmortem mRNA studies

Monika Cs Horvath, Yasmin L. Hurd, Jovan Rajs, E. Keller

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: To determine whether there are factors during apparent rapid heroin overdose death that affect agonal state and thus brain pH (index of hypoxia) that can influence neurobiological systems linked to drug abuse. Design and methods: Brain specimens and autopsy/medical reports were investigated in subjects dying from heroin overdose (n = 70) and compared to normal controls (n = 45) as well as suicide victims (n = 31) with a documented rapid cause of death. Detailed autopsy material was characterized as to positive and negative respiratory distress in relation to brain pH; drug toxicity and other demographic information was also evaluated. In situ hybridization histochemistry was used to study mRNA expression levels of dopamine (e.g., D2 receptor, dopamine transporter) and opioid (e.g., proenkephalin) related markers in various structures in relation to brain pH. Findings: Brain pH was generally reduced in heroin overdose cases versus normal and suicide subjects. There was, however, significant variation in heroin overdose deaths related to differences in respiratory distress that differentially altered brain pH levels. Various factors such as vomit inhalation, resuscitation, pulmonary embolism and suffocation contributed to positive respiratory distress. Elevated brain pH was observed in heroin overdose with positive alcohol toxicity suggesting potentiated alcohol-induced rapidity of heroin deaths. mRNA expression levels of the dopamine-related genes and proenkephalin were positively correlated with brain pH. Conclusions: Respiratory distress contributes to variations in the acute agonal state during heroin overdose death that differentially alters brain pH levels and significantly impacts mRNA levels. Such findings should be considered for postmortem molecular/neurochemical neurobiological studies of opiate abusers.

Original languageEnglish
Pages (from-to)251-259
Number of pages9
JournalBrain Research Bulletin
Volume70
Issue number3
DOIs
Publication statusPublished - Jul 31 2006

Fingerprint

Heroin
Messenger RNA
Brain
Suicide
Opiate Alkaloids
Autopsy
Alcohols
Dopamine Plasma Membrane Transport Proteins
Dopamine D2 Receptors
Asphyxia
Drug-Related Side Effects and Adverse Reactions
Pulmonary Embolism
Resuscitation
Inhalation
Opioid Analgesics
Substance-Related Disorders
In Situ Hybridization
Cause of Death
Dopamine
Demography

Keywords

  • Brain pH
  • Dopamine
  • Opiate abuse
  • Opioid neuropeptide
  • Suicide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Variations in respiratory distress characterize the acute agonal period during heroin overdose death : Relevance to postmortem mRNA studies. / Horvath, Monika Cs; Hurd, Yasmin L.; Rajs, Jovan; Keller, E.

In: Brain Research Bulletin, Vol. 70, No. 3, 31.07.2006, p. 251-259.

Research output: Contribution to journalArticle

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