Variation of NimC1 expression in Drosophila stocks and transgenic strains

Viktor Honti, Gyöngyi Cinege, Gábor Csordás, E. Kurucz, János Zsámboki, Cory J. Eevans, Utpal Banerjee, I. Andó

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The NimC1 molecule has been described as a phagocytosis receptor, and is being used as a marker for professional phagocytes, the plasmatocytes, in Drosophila melanogaster. In studies including tumor-biology, developmental biology, and cell mediated immunity, monoclonal antibodies (P1a and P1b) to the NimC1 antigen are used. Aas we observed that these antibodies did not react with plasmatocytes of several strains and genetic combinations, a molecular analysis was performed on the structure of the nimC1 gene. In these strains we found 2 deletions and an insertion within the nimC1 gene, which may result in the production of a truncated NimC1 protein. The NimC1 positivity was regained by recombining the mutation with a wild-type allele or by using nimC1 mutant lines under heterozygous conditions. By means of these procedures or using the recombined stock, NimC1 can be used as a marker for phagocytic cells in the majority of the possible genetic backgrounds.

Original languageEnglish
JournalFly
Volume7
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

phagocytes
Phagocytes
Drosophila
genetically modified organisms
Developmental Biology
Biological Sciences
Drosophila melanogaster
phagocytosis
Cellular Immunity
cell-mediated immunity
genetic background
Genes
monoclonal antibodies
genes
Alleles
Monoclonal Antibodies
antigens
alleles
mutation
Antigens

Keywords

  • Drosophila
  • Innate immunity
  • Marker
  • Phagocytosis receptor
  • Plasmatocyte

ASJC Scopus subject areas

  • Insect Science
  • Medicine(all)

Cite this

Variation of NimC1 expression in Drosophila stocks and transgenic strains. / Honti, Viktor; Cinege, Gyöngyi; Csordás, Gábor; Kurucz, E.; Zsámboki, János; Eevans, Cory J.; Banerjee, Utpal; Andó, I.

In: Fly, Vol. 7, No. 4, 2013.

Research output: Contribution to journalArticle

Honti, V, Cinege, G, Csordás, G, Kurucz, E, Zsámboki, J, Eevans, CJ, Banerjee, U & Andó, I 2013, 'Variation of NimC1 expression in Drosophila stocks and transgenic strains', Fly, vol. 7, no. 4. https://doi.org/10.4161/fly.25654
Honti, Viktor ; Cinege, Gyöngyi ; Csordás, Gábor ; Kurucz, E. ; Zsámboki, János ; Eevans, Cory J. ; Banerjee, Utpal ; Andó, I. / Variation of NimC1 expression in Drosophila stocks and transgenic strains. In: Fly. 2013 ; Vol. 7, No. 4.
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AU - Eevans, Cory J.

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