Variation of isoenzyme and RAPD patterns in Candida albicans morphological mutants with altered colony ultrastructure

M. Pesti, C. Vágvölgyi, T. Papp, A. Nagy, A. Novák

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Molecular typing methods were applied to characterize four stable morphological mutants [1] isolated from a UV-induced unstable mutant colony of Candida albicans. The wild-type strain (ATCC 64385), the intermediate unstable mutant and its four morphologically altered derivatives revealed the same electrophoretic karyotypes. Of the five isoenzymes tested (catalase, malate dehydrogenase, glutamate dehydrogenase, acid phosphatase and β-glucosidase), glutamate dehydrogenase displayed a different enzyme pattern (with an extra band of lower mobility) in the morphological mutants. In contrast, the random amplification DNA polymorphism patterns of the mutant strains differed in all cases from that of the parental strain. Different primers revealed various degrees of DNA polymorphism; one of them (OPC-8) proved to be useful for differentiation between all examined strains. Differences in genetic alterations between spontaneous and induced mutants, and the applicability of different molecular markers to analyse the consequences of induced mutagenesis in C. albicans are discussed.

Original languageEnglish
Pages (from-to)289-298
Number of pages10
JournalActa Biologica Hungarica
Volume52
Issue number2-3
DOIs
Publication statusPublished - 2001

Fingerprint

Glutamate Dehydrogenase
Candida
ultrastructure
Candida albicans
Isoenzymes
isozymes
Glucosidases
Polymorphism
Molecular Typing
mutants
Malate Dehydrogenase
DNA
Acid Phosphatase
Karyotype
Mutagenesis
Catalase
glutamate dehydrogenase
Amplification
polymorphism
Enzymes

Keywords

  • Candida
  • Morphogenesis
  • RAPD

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Variation of isoenzyme and RAPD patterns in Candida albicans morphological mutants with altered colony ultrastructure. / Pesti, M.; Vágvölgyi, C.; Papp, T.; Nagy, A.; Novák, A.

In: Acta Biologica Hungarica, Vol. 52, No. 2-3, 2001, p. 289-298.

Research output: Contribution to journalArticle

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