Variability of bioactive glucosinolates, isothiocyanates and enzyme patterns in horseradish hairy root cultures initiated from different organs

Regina Bertóti, Andrea Böszörményi, Ágnes Alberti, S. Béni, Márta M-Hamvas, E. Szőke, G. Vasas, Sándor Gonda

Research output: Contribution to journalArticle

Abstract

Horseradish hairy root cultures are suitable plant tissue organs to study the glucosinolate–myrosinase–isothiocyanate system and also to produce the biologically active isothiocyanates and horseradish peroxidase, widely used in molecular biology. Fifty hairy root clones were isolated after Agrobacterium rhizogenes infection of surface sterilized Armoracia rusticana petioles and leaf blades, from which 21 were viable after antibiotic treatment. Biomass properties (e.g. dry weight %, daily growth index), glucosinolate content (analyzed by liquid chromatography-electronspray ionization-mass spectrometry (LC-ESI-MS/MS)), isothiocyanate and nitrile content (analyzed by gas chromatography-mass spectrometry (GC-MS)), myrosinase (on-gel detection) and horseradish peroxidase enzyme patterns (on-gel detection and spectrophotometry), and morphological features were examined with multi-variable statistical analysis. In addition to the several positive and negative correlations, the most outstanding phenomenon was many parameters of the hairy root clones showed dependence on the organ of origin. Among others, the daily growth index, sinigrin, glucobrassicin, 3-phenylpropionitrile, indole-3-acetonitrile and horseradish peroxidase values showed significantly higher levels in horseradish hairy root cultures initiated from leaf blades.

Original languageEnglish
Article number2828
JournalMolecules
Volume24
Issue number15
DOIs
Publication statusPublished - Aug 2 2019

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Armoracia
Isothiocyanates
Glucosinolates
Horseradish Peroxidase
activity (biology)
blades
organs
leaves
enzymes
mass spectroscopy
gels
molecular biology
Mass spectrometry
antibiotics
nitriles
indoles
liquid chromatography
infectious diseases
Enzymes
spectrophotometry

Keywords

  • Glucosinolate
  • Hairy root
  • Horseradish
  • Horseradish peroxidase
  • Isothiocyanate
  • Myrosinase
  • Nitrile

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Variability of bioactive glucosinolates, isothiocyanates and enzyme patterns in horseradish hairy root cultures initiated from different organs. / Bertóti, Regina; Böszörményi, Andrea; Alberti, Ágnes; Béni, S.; M-Hamvas, Márta; Szőke, E.; Vasas, G.; Gonda, Sándor.

In: Molecules, Vol. 24, No. 15, 2828, 02.08.2019.

Research output: Contribution to journalArticle

Bertóti, Regina ; Böszörményi, Andrea ; Alberti, Ágnes ; Béni, S. ; M-Hamvas, Márta ; Szőke, E. ; Vasas, G. ; Gonda, Sándor. / Variability of bioactive glucosinolates, isothiocyanates and enzyme patterns in horseradish hairy root cultures initiated from different organs. In: Molecules. 2019 ; Vol. 24, No. 15.
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