Variability in xylanase and xylanase inhibition activities in different cereals in the HEALTHGRAIN diversity screen and contribution of environment and genotype to this variability in common wheat

Kurt Gebruers, Emmie Dornez, Z. Bedő, M. Rakszegi, Christophe M. Courtin, Jan A. Delcour

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Endo-1,4-β-d-xylanases (EC 3.2.1.8, xylanases) and xylanase inhibitors, that is, TAXI (Triticum aestivum xylanase inhibitor), XIP (xylanase inhibiting protein), and TLXI (thaumatin-like xylanase inhibitor) type xylanase inhibitors, which naturally occur in cereals, are believed to be at the basis of a significant part of the variability in biotechnological functional properties of cereals. Xylanase inhibitors in particular affect grain functionality during processing and in animal feeds when xylanases are used to improve processing parameters and product quality. In the present study the variability of xylanase, TAXI, and XIP activities was quantified in different cereals, including different wheat types [common wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), spelt wheat (Triticum spelta L.), einkorn wheat (Triticum monococcum L.), and emmer wheat (Triticum dicoccum Schübler)], barley (Hordeum vulgare L.), rye (Secale cereale L.), and oat (Avena sativa L.), and the contribution of genotype and environment to this variability in common wheat was estimated. Substantial differences in xylanase, TAXI, and XIP activities exist between the different cereal types and varieties. Under the experimental conditions of this study, the durum wheat samples show very high xylanase activities compared to the other cereals. High TAXI and XIP activities were measured in, for example, common wheat, spelt wheat, and rye, whereas low activities occur in barley and oat. For wheat, a significant part of the variability in inhibitor levels can be explained by genotype, whereas xylanase activity is most strongly determined by environment. The results obtained suggest that plant breeders and industry to certain extent can select for wheat varieties with high or low xylanase inhibition activities, but the relatively high contribution of the genotype-environment interaction term to the total variability in inhibition activities indicates that TAXI and XIP activities are not very stable breeding parameters.

Original languageEnglish
Pages (from-to)9362-9371
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number17
DOIs
Publication statusPublished - Sep 8 2010

Fingerprint

xylanases
Triticum
Genotype
wheat
genotype
Proteins
Triticum aestivum
Processing
Triticum aestivum subsp. spelta
Animals
Edible Grain
Hordeum
Triticum turgidum subsp. dicoccon
durum wheat
proteins
rye
Industry
oats
barley
endo-1,4-beta-xylanase

Keywords

  • Cereals
  • inhibitor
  • TAXI
  • wheat
  • XIP
  • xylanase

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

@article{565b2d6ee0ab4ceebb02dc059e97eff7,
title = "Variability in xylanase and xylanase inhibition activities in different cereals in the HEALTHGRAIN diversity screen and contribution of environment and genotype to this variability in common wheat",
abstract = "Endo-1,4-β-d-xylanases (EC 3.2.1.8, xylanases) and xylanase inhibitors, that is, TAXI (Triticum aestivum xylanase inhibitor), XIP (xylanase inhibiting protein), and TLXI (thaumatin-like xylanase inhibitor) type xylanase inhibitors, which naturally occur in cereals, are believed to be at the basis of a significant part of the variability in biotechnological functional properties of cereals. Xylanase inhibitors in particular affect grain functionality during processing and in animal feeds when xylanases are used to improve processing parameters and product quality. In the present study the variability of xylanase, TAXI, and XIP activities was quantified in different cereals, including different wheat types [common wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), spelt wheat (Triticum spelta L.), einkorn wheat (Triticum monococcum L.), and emmer wheat (Triticum dicoccum Sch{\"u}bler)], barley (Hordeum vulgare L.), rye (Secale cereale L.), and oat (Avena sativa L.), and the contribution of genotype and environment to this variability in common wheat was estimated. Substantial differences in xylanase, TAXI, and XIP activities exist between the different cereal types and varieties. Under the experimental conditions of this study, the durum wheat samples show very high xylanase activities compared to the other cereals. High TAXI and XIP activities were measured in, for example, common wheat, spelt wheat, and rye, whereas low activities occur in barley and oat. For wheat, a significant part of the variability in inhibitor levels can be explained by genotype, whereas xylanase activity is most strongly determined by environment. The results obtained suggest that plant breeders and industry to certain extent can select for wheat varieties with high or low xylanase inhibition activities, but the relatively high contribution of the genotype-environment interaction term to the total variability in inhibition activities indicates that TAXI and XIP activities are not very stable breeding parameters.",
keywords = "Cereals, inhibitor, TAXI, wheat, XIP, xylanase",
author = "Kurt Gebruers and Emmie Dornez and Z. Bedő and M. Rakszegi and Courtin, {Christophe M.} and Delcour, {Jan A.}",
year = "2010",
month = "9",
day = "8",
doi = "10.1021/jf100474m",
language = "English",
volume = "58",
pages = "9362--9371",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "17",

}

TY - JOUR

T1 - Variability in xylanase and xylanase inhibition activities in different cereals in the HEALTHGRAIN diversity screen and contribution of environment and genotype to this variability in common wheat

AU - Gebruers, Kurt

AU - Dornez, Emmie

AU - Bedő, Z.

AU - Rakszegi, M.

AU - Courtin, Christophe M.

AU - Delcour, Jan A.

PY - 2010/9/8

Y1 - 2010/9/8

N2 - Endo-1,4-β-d-xylanases (EC 3.2.1.8, xylanases) and xylanase inhibitors, that is, TAXI (Triticum aestivum xylanase inhibitor), XIP (xylanase inhibiting protein), and TLXI (thaumatin-like xylanase inhibitor) type xylanase inhibitors, which naturally occur in cereals, are believed to be at the basis of a significant part of the variability in biotechnological functional properties of cereals. Xylanase inhibitors in particular affect grain functionality during processing and in animal feeds when xylanases are used to improve processing parameters and product quality. In the present study the variability of xylanase, TAXI, and XIP activities was quantified in different cereals, including different wheat types [common wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), spelt wheat (Triticum spelta L.), einkorn wheat (Triticum monococcum L.), and emmer wheat (Triticum dicoccum Schübler)], barley (Hordeum vulgare L.), rye (Secale cereale L.), and oat (Avena sativa L.), and the contribution of genotype and environment to this variability in common wheat was estimated. Substantial differences in xylanase, TAXI, and XIP activities exist between the different cereal types and varieties. Under the experimental conditions of this study, the durum wheat samples show very high xylanase activities compared to the other cereals. High TAXI and XIP activities were measured in, for example, common wheat, spelt wheat, and rye, whereas low activities occur in barley and oat. For wheat, a significant part of the variability in inhibitor levels can be explained by genotype, whereas xylanase activity is most strongly determined by environment. The results obtained suggest that plant breeders and industry to certain extent can select for wheat varieties with high or low xylanase inhibition activities, but the relatively high contribution of the genotype-environment interaction term to the total variability in inhibition activities indicates that TAXI and XIP activities are not very stable breeding parameters.

AB - Endo-1,4-β-d-xylanases (EC 3.2.1.8, xylanases) and xylanase inhibitors, that is, TAXI (Triticum aestivum xylanase inhibitor), XIP (xylanase inhibiting protein), and TLXI (thaumatin-like xylanase inhibitor) type xylanase inhibitors, which naturally occur in cereals, are believed to be at the basis of a significant part of the variability in biotechnological functional properties of cereals. Xylanase inhibitors in particular affect grain functionality during processing and in animal feeds when xylanases are used to improve processing parameters and product quality. In the present study the variability of xylanase, TAXI, and XIP activities was quantified in different cereals, including different wheat types [common wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), spelt wheat (Triticum spelta L.), einkorn wheat (Triticum monococcum L.), and emmer wheat (Triticum dicoccum Schübler)], barley (Hordeum vulgare L.), rye (Secale cereale L.), and oat (Avena sativa L.), and the contribution of genotype and environment to this variability in common wheat was estimated. Substantial differences in xylanase, TAXI, and XIP activities exist between the different cereal types and varieties. Under the experimental conditions of this study, the durum wheat samples show very high xylanase activities compared to the other cereals. High TAXI and XIP activities were measured in, for example, common wheat, spelt wheat, and rye, whereas low activities occur in barley and oat. For wheat, a significant part of the variability in inhibitor levels can be explained by genotype, whereas xylanase activity is most strongly determined by environment. The results obtained suggest that plant breeders and industry to certain extent can select for wheat varieties with high or low xylanase inhibition activities, but the relatively high contribution of the genotype-environment interaction term to the total variability in inhibition activities indicates that TAXI and XIP activities are not very stable breeding parameters.

KW - Cereals

KW - inhibitor

KW - TAXI

KW - wheat

KW - XIP

KW - xylanase

UR - http://www.scopus.com/inward/record.url?scp=77954404622&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954404622&partnerID=8YFLogxK

U2 - 10.1021/jf100474m

DO - 10.1021/jf100474m

M3 - Article

C2 - 20462210

AN - SCOPUS:77954404622

VL - 58

SP - 9362

EP - 9371

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 17

ER -