Vanilloid receptors in hearing: Altered cochlear sensitivity by vanilloids and expression of TRPV1 in the organ of Corti

Jiefu Zheng, Chunfu Dai, Peter S. Steyger, Youngki Kim, Z. Vass, Tianying Ren, Alfred L. Nuttall

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Capsaicin, the vanilloid that selectively activates vanilloid receptors (VRs) on sensory neurons for noxious perception, has been reported to increase cochlear blood flow (CBF). VR-related receptors have also been found in the inner ear. This study aims to address the question as to whether VRs exist in the organ of Corti and play a role in cochlear physiology. Capsaicin or the more potent VR agonist, resiniferatoxin (RTX), was infused into the scala tympani of guinea pig cochlea, and their effects on cochlear sensitivity were investigated. Capsaicin (20 μM) elevated the threshold of auditory nerve compound action potential and reduced the magnitude of cochlear microphonic and electrically evoked otoacoustic emissions. These effects were reversible and could be blocked by a competitive antagonist, capsazepine. Application of 2 μM RTX resulted in cochlear sensitivity alterations similar to that by capsaicin, which could also be blocked by capsazepine. A desensitization phenomenon was observed in the case of prolonged perfusion with either capsaicin or RTX. Brief increase of CBF by capsaicin was confirmed, and the endocochlear potential was not decreased. Basilar membrane velocity (BM) growth functions near the best frequency and BM tuning were altered by capsaicin. Immunohistochemistry study revealed the presence of vanilloid receptor type 1 of the transient receptor potential channel family in the hair cells and supporting cells of the organ of Corti and the spiral ganglion cells of the cochlea. The results indicate that the main action of capsaicin is on outer hair cells and suggest that VRs in the cochlea play a role in cochlear homeostasis.

Original languageEnglish
Pages (from-to)444-455
Number of pages12
JournalJournal of Neurophysiology
Volume90
Issue number1
Publication statusPublished - Jul 1 2003

Fingerprint

TRPV Cation Channels
Organ of Corti
Cochlea
Hearing
Capsaicin
Basilar Membrane
Outer Auditory Hair Cells
Scala Tympani
Spiral Ganglion
Transient Receptor Potential Channels
Cochlear Nerve
Sensory Receptor Cells
Inner Ear
Action Potentials
Guinea Pigs
Homeostasis
Perfusion
Immunohistochemistry

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Vanilloid receptors in hearing : Altered cochlear sensitivity by vanilloids and expression of TRPV1 in the organ of Corti. / Zheng, Jiefu; Dai, Chunfu; Steyger, Peter S.; Kim, Youngki; Vass, Z.; Ren, Tianying; Nuttall, Alfred L.

In: Journal of Neurophysiology, Vol. 90, No. 1, 01.07.2003, p. 444-455.

Research output: Contribution to journalArticle

Zheng, Jiefu ; Dai, Chunfu ; Steyger, Peter S. ; Kim, Youngki ; Vass, Z. ; Ren, Tianying ; Nuttall, Alfred L. / Vanilloid receptors in hearing : Altered cochlear sensitivity by vanilloids and expression of TRPV1 in the organ of Corti. In: Journal of Neurophysiology. 2003 ; Vol. 90, No. 1. pp. 444-455.
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