Validity of selected DNA markers for breeding leaf rust resistant wheat

L. Błaszczyk, I. Kramer, F. Ordon, J. Chełkowski, M. Tyrka, G. Vida, I. Karsai

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The set of 44 near-isogenic lines of spring wheat cv. Thatcher and wheat genotypes known to carry specific leaf rust resistance genes were included in the present study for the preliminary validation of twelve STS and SCAR markers linked to leaf rust resistance genes Lr9, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr37. Seven Lr genes were specifically tagged by STS and SCAR markers. The presence of genes Lr9, Lr19, Lr20, Lr24, Lr28, Lr29, Lr37 in the tested plant materials was confirmed by a unique amplification of markers SCS5550, SCS265512 and SCS253736, STS638, SCS73719, SCS421570, IPY10 and Lr29F24/R24, cslVrgal3, PS10R/L, respectively. Evaluation of the repeatability and the reliability of selected markers (pTAG621 for Lr1, STS683 for Lr20, D14L for Lr21, Lr25F20/R19 for Lr25, Lr29F24/R24, IPY10 for Lr29, cslVrgal3 for Lr37 and PS10R/L for Lr47) across four European laboratories and PCR conditions demonstrated the usability of STS638, Lr29F24/R24, IPY10, cslVrgal3 and PS10R/L markers in marker-assisted selection. STS markers pTAG621 for gene Lr1, D14L for gene Lr21, Lr25F20/R19 for gene Lr25 were found to be unsuitable for effective screening of large segregating populations in breeding programs.

Original languageEnglish
Pages (from-to)201-213
Number of pages13
JournalCereal Research Communications
Volume36
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

leaf rust
Genetic Markers
Triticum
Breeding
wheat
genetic markers
breeding
Genes
genes
isogenic lines
spring wheat
Validation Studies
marker-assisted selection
repeatability
screening
Genotype
genotype
Polymerase Chain Reaction
Population

Keywords

  • DNA markers
  • Leaf rust
  • Resistance genes
  • Wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Physiology

Cite this

Błaszczyk, L., Kramer, I., Ordon, F., Chełkowski, J., Tyrka, M., Vida, G., & Karsai, I. (2008). Validity of selected DNA markers for breeding leaf rust resistant wheat. Cereal Research Communications, 36(2), 201-213. https://doi.org/10.1556/CRC.36.2008.2.1

Validity of selected DNA markers for breeding leaf rust resistant wheat. / Błaszczyk, L.; Kramer, I.; Ordon, F.; Chełkowski, J.; Tyrka, M.; Vida, G.; Karsai, I.

In: Cereal Research Communications, Vol. 36, No. 2, 2008, p. 201-213.

Research output: Contribution to journalArticle

Błaszczyk, L, Kramer, I, Ordon, F, Chełkowski, J, Tyrka, M, Vida, G & Karsai, I 2008, 'Validity of selected DNA markers for breeding leaf rust resistant wheat', Cereal Research Communications, vol. 36, no. 2, pp. 201-213. https://doi.org/10.1556/CRC.36.2008.2.1
Błaszczyk L, Kramer I, Ordon F, Chełkowski J, Tyrka M, Vida G et al. Validity of selected DNA markers for breeding leaf rust resistant wheat. Cereal Research Communications. 2008;36(2):201-213. https://doi.org/10.1556/CRC.36.2008.2.1
Błaszczyk, L. ; Kramer, I. ; Ordon, F. ; Chełkowski, J. ; Tyrka, M. ; Vida, G. ; Karsai, I. / Validity of selected DNA markers for breeding leaf rust resistant wheat. In: Cereal Research Communications. 2008 ; Vol. 36, No. 2. pp. 201-213.
@article{c081e02287154a4d81d93ed13db6067c,
title = "Validity of selected DNA markers for breeding leaf rust resistant wheat",
abstract = "The set of 44 near-isogenic lines of spring wheat cv. Thatcher and wheat genotypes known to carry specific leaf rust resistance genes were included in the present study for the preliminary validation of twelve STS and SCAR markers linked to leaf rust resistance genes Lr9, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr37. Seven Lr genes were specifically tagged by STS and SCAR markers. The presence of genes Lr9, Lr19, Lr20, Lr24, Lr28, Lr29, Lr37 in the tested plant materials was confirmed by a unique amplification of markers SCS5550, SCS265512 and SCS253736, STS638, SCS73719, SCS421570, IPY10 and Lr29F24/R24, cslVrgal3, PS10R/L, respectively. Evaluation of the repeatability and the reliability of selected markers (pTAG621 for Lr1, STS683 for Lr20, D14L for Lr21, Lr25F20/R19 for Lr25, Lr29F24/R24, IPY10 for Lr29, cslVrgal3 for Lr37 and PS10R/L for Lr47) across four European laboratories and PCR conditions demonstrated the usability of STS638, Lr29F24/R24, IPY10, cslVrgal3 and PS10R/L markers in marker-assisted selection. STS markers pTAG621 for gene Lr1, D14L for gene Lr21, Lr25F20/R19 for gene Lr25 were found to be unsuitable for effective screening of large segregating populations in breeding programs.",
keywords = "DNA markers, Leaf rust, Resistance genes, Wheat",
author = "L. Błaszczyk and I. Kramer and F. Ordon and J. Chełkowski and M. Tyrka and G. Vida and I. Karsai",
year = "2008",
doi = "10.1556/CRC.36.2008.2.1",
language = "English",
volume = "36",
pages = "201--213",
journal = "Cereal Research Communications",
issn = "0133-3720",
publisher = "Cereal Research Non-Profit Company",
number = "2",

}

TY - JOUR

T1 - Validity of selected DNA markers for breeding leaf rust resistant wheat

AU - Błaszczyk, L.

AU - Kramer, I.

AU - Ordon, F.

AU - Chełkowski, J.

AU - Tyrka, M.

AU - Vida, G.

AU - Karsai, I.

PY - 2008

Y1 - 2008

N2 - The set of 44 near-isogenic lines of spring wheat cv. Thatcher and wheat genotypes known to carry specific leaf rust resistance genes were included in the present study for the preliminary validation of twelve STS and SCAR markers linked to leaf rust resistance genes Lr9, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr37. Seven Lr genes were specifically tagged by STS and SCAR markers. The presence of genes Lr9, Lr19, Lr20, Lr24, Lr28, Lr29, Lr37 in the tested plant materials was confirmed by a unique amplification of markers SCS5550, SCS265512 and SCS253736, STS638, SCS73719, SCS421570, IPY10 and Lr29F24/R24, cslVrgal3, PS10R/L, respectively. Evaluation of the repeatability and the reliability of selected markers (pTAG621 for Lr1, STS683 for Lr20, D14L for Lr21, Lr25F20/R19 for Lr25, Lr29F24/R24, IPY10 for Lr29, cslVrgal3 for Lr37 and PS10R/L for Lr47) across four European laboratories and PCR conditions demonstrated the usability of STS638, Lr29F24/R24, IPY10, cslVrgal3 and PS10R/L markers in marker-assisted selection. STS markers pTAG621 for gene Lr1, D14L for gene Lr21, Lr25F20/R19 for gene Lr25 were found to be unsuitable for effective screening of large segregating populations in breeding programs.

AB - The set of 44 near-isogenic lines of spring wheat cv. Thatcher and wheat genotypes known to carry specific leaf rust resistance genes were included in the present study for the preliminary validation of twelve STS and SCAR markers linked to leaf rust resistance genes Lr9, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr28, Lr29, Lr37. Seven Lr genes were specifically tagged by STS and SCAR markers. The presence of genes Lr9, Lr19, Lr20, Lr24, Lr28, Lr29, Lr37 in the tested plant materials was confirmed by a unique amplification of markers SCS5550, SCS265512 and SCS253736, STS638, SCS73719, SCS421570, IPY10 and Lr29F24/R24, cslVrgal3, PS10R/L, respectively. Evaluation of the repeatability and the reliability of selected markers (pTAG621 for Lr1, STS683 for Lr20, D14L for Lr21, Lr25F20/R19 for Lr25, Lr29F24/R24, IPY10 for Lr29, cslVrgal3 for Lr37 and PS10R/L for Lr47) across four European laboratories and PCR conditions demonstrated the usability of STS638, Lr29F24/R24, IPY10, cslVrgal3 and PS10R/L markers in marker-assisted selection. STS markers pTAG621 for gene Lr1, D14L for gene Lr21, Lr25F20/R19 for gene Lr25 were found to be unsuitable for effective screening of large segregating populations in breeding programs.

KW - DNA markers

KW - Leaf rust

KW - Resistance genes

KW - Wheat

UR - http://www.scopus.com/inward/record.url?scp=52649119718&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52649119718&partnerID=8YFLogxK

U2 - 10.1556/CRC.36.2008.2.1

DO - 10.1556/CRC.36.2008.2.1

M3 - Article

VL - 36

SP - 201

EP - 213

JO - Cereal Research Communications

JF - Cereal Research Communications

SN - 0133-3720

IS - 2

ER -