Validation of a densitometric method for the determination of theanine in tea extracts using CP atlas software

Dezsára Boros, Attila Hunyadi, Katalin Veres, J. Hohmann

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

L-Theanine is a non-protein amino acid that occurs in the leaves of the tea plant (Camellia sinensis) and possesses several pharmacologic effects, and therefore it is widely applied in the food industry. Considering the chemical characteristics of the molecule (high polarity, lack of chromophore group), conventional HPLC-based methods are not optimal for the quantification of the compound. However, for TLC chromatographic separation of theanine in tea extracts, there are reliable methods available and TLC analysis allows derivatization for better detection of the compound. Here we report for the first time the development and validation of an eligible densitometric method based on the analysis of digital photographs of TLC plates without the need of densitometer and using a software available free of charge for the quick and reliable determination of theanine in tea extracts.

Original languageEnglish
Pages (from-to)571-574
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume25
Issue number6
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

Atlases
Tea
Software
Camellia sinensis
Densitometers
Plant Leaves
Food Industry
Chromophores
High Pressure Liquid Chromatography
Amino Acids
Molecules
theanine
Industry

Keywords

  • Camellia sinensis L.
  • CP Atlas
  • Densitometry
  • Theanine
  • TLC
  • Validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Validation of a densitometric method for the determination of theanine in tea extracts using CP atlas software. / Boros, Dezsára; Hunyadi, Attila; Veres, Katalin; Hohmann, J.

In: Journal of Planar Chromatography - Modern TLC, Vol. 25, No. 6, 01.12.2012, p. 571-574.

Research output: Contribution to journalArticle

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