A simple and sensitive high-performance liquid chromatographic method for the determination of ciprofloxacin in influenza vaccine has been developed. The stationary phase was a Purospher RP-18e column (125 × 3.5 mm; 5 μm), the mobile phase consisted of acetonitrile/water/phosphoric acid (85%) (15 : 85 : 0.25, v/v/v %), and its pH was adjusted to pH 3.00 using distilled triethylamine immediately before use. Separation was achieved using a flow rate of 0.6 mL/min at ambient temperature. The ciprofloxacin was detected at 280 nm. The retention time for ciprofloxacin was 4.60 ± 0.15 min. The limit of detection was 2 ng/mL; the limit of quantification was found to be 5 ng/mL.
|Number of pages||10|
|Journal||Journal of Liquid Chromatography and Related Technologies|
|Publication status||Published - 2001|
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Clinical Biochemistry