Validated determination of ciprofloxacin in influenza vaccine by RP-HPLC

P. Forlay-Frick, Z. B. Nagy, J. Fekete

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A simple and sensitive high-performance liquid chromatographic method for the determination of ciprofloxacin in influenza vaccine has been developed. The stationary phase was a Purospher RP-18e column (125 × 3.5 mm; 5 μm), the mobile phase consisted of acetonitrile/water/phosphoric acid (85%) (15 : 85 : 0.25, v/v/v %), and its pH was adjusted to pH 3.00 using distilled triethylamine immediately before use. Separation was achieved using a flow rate of 0.6 mL/min at ambient temperature. The ciprofloxacin was detected at 280 nm. The retention time for ciprofloxacin was 4.60 ± 0.15 min. The limit of detection was 2 ng/mL; the limit of quantification was found to be 5 ng/mL.

Original languageEnglish
Pages (from-to)827-836
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Volume24
Issue number6
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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